Diagenode

BCL6 polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15410221-100
100 μl
$345.00
  Bulk order

Polyclonal antibody raised in rabbit against BCL6 (B-cell CLL/lymphoma 6), using a recombinant protein.

Lot40163
Concentration1 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP 5 μg/ChIP Fig 5
IHC 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:100 - 1:500 Fig 1
Western Blotting 1:500 - 1:3,000 Fig 2, 3
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    Immunoprecipitation

    Figure 1. Immunoprecipitation
    BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell extract A: 30 μg whole cell extract of BCL6 protein expressing Raji cells B: Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody. The immunoprecipitated BCL6 protein was detected by Western blot with the BCL6 antibody diluted 1:1,000.

    Western blot

    Figure 2. Western blot results
    Sample: 30 μg of whole cell lysate A: Jurkat B: Raji C: NCI-H929 7.5% SDS PAGE BCL6 antibody diluted 1:1,000

    Western blot

    Figure 3. Western blot results
    BCL6 antibody detects BCL6 protein by Western blot analysis. A. 30 μg Neuro2A whole cell extract B. 30 μg GL261 whole cell extract C. 30 μg C8D30 whole cell extract D. 30 μg NIH-3T3 whole cell extract E. 30 μg Raw264.7 whole cell extract F. 30 μg C2C12 whole cell extract 7.5 % SDS-PAGE The BCL6 antibody (Cat. No. C15410221) was used at a dilution of 1:1,000

    IHC

    Figure 4. IHC
    Immunohistochemical analysis of paraffin-embedded Tonsil, using BCL6 (C15410221) antibody (10 μg/ml).

    ChIP

    Figure 5. ChIP
    ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.Results are expressed as fold enrichment over the IgG negative control.

  •  実験手法
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  資料
    Datasheet BCL6 C15410221 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  出版物

    How to properly cite this product in your work

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  •  関連商品

イベント

  • ASHG
    Houston, TX
    Oct 15-Oct 19, 2019
  • ddd
    dd
    Oct 18-Oct 26, 2019
  • Neuroscience 2019
    Chicago, IL
    Oct 19-Oct 23, 2019
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