Protocols
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Download Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation
Histology-defined proteomics investigations are a fundamental tool in modern molecular pathology. Molecular signatures of specific cells must be detected within their correct histopathological cont... -
Download iST Sample Preparation with the Bioruptor®
The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses. -
Download Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing
Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation of most archived pathological specimens. Archival FFPE samples provide an invaluable repository of in... -
Download Transfection of CRISPR/Cas9 Nuclease NLS ribonucleoprotein (RNP) into adherent mammalian cells using Lipofectamine® RNAiMAX
The CRISPR/Cas genome editing system consists of a single guide RNA (sgRNA) and the Cas9 endonuclease. To create gene disruptions, a single guide RNA is generated to direct the Cas9 nuclease to a s... -
Download CRISPR/Cas9 editing: mutation detection with mismatch cleavage assay
Genome editing using CRISPR/Cas9 is used for targeted mutagenesis. But because genome editing does not target all loci with similar efficiencies, the mutation hit-rate at a given locus needs to be ... -
Download DNA shearing for Next-Generation Sequencing with the Bioruptor® Pico (2013-2019), 0.2 ml Bioruptor® microtubes and the corresponding holder
Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, among others the precise DNA fragmentation. The Diagenode Bioruptor® Pico ensures that the DNA is e... -
Download Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder
The first critical step of a successful ChIP experiment is the preparation of sheared chromatin with a suitable fragment range which best represents the biological scenario being investigated. The ... -
Download Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico
ChIP-seq is a common tool used to study protein-DNA interactions in cells and tissues on a genome-wide scale. The size of the immunoprecipitated DNA fragments is a key parameter for successful libr... -
Download Mass-Spectrometry: procedure for shotgun proteomics
Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in add... -
Download Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis
Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck Institute for Biochemistry, Martinsried, Germany are using the Bioruptor® for their research usi... -
Download Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®
Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene, or a mineral oil-based method which can be time consuming and messy. Claire Josse and colleagues f... -
Download TruSeq™ ChIP Sample Preparation - IP-Star® Compact
The “TruSeq ChIP SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ ChIP Sample Preparation” kit and reagents from Illumina®. It provides flex... -
Download TruSeq™ Stranded RNA Sample Preparation - IP-Star® Compact
The “TruSeq Stranded RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ Stranded Total RNA Sample Preparation” kit or “TruSeq™ Str... -
Download iDeal Library Preparation kit - IP-Star® compact
The “iDeal Library Preparation” protocol on the IP-Star® is using the standard “Ideal Library Preparation kit” and reagents from Diagenode. The Ideal Library Preparation... -
Download TruSeq™ RNA Sample Preparation - IP-Star Compact
The “TruSeq RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ RNA Sample Preparation” kit v2 and reagents from Illumina®. It provides fle... -
Download AMPure® XP Size Selection - IP-Star Compact
The “AMPure XP Size Selection” protocol on the IP-Star® is using the standard “Agencourt® AMPure® XP Beads” from Agencourt®. It allows you to perform size se... -
Download TruSeq™ DNA PCR-Free Sample Preparation - IP-Star Compact
The “TruSeq™ DNA PCR-Free SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ DNA PCR-Free Sample Preparation” kit and reagents from Illumina... -
Download NEBNext® ChIP-Seq Library Prep for Illumina - IP-Star Compact
The “NEBNext ChIP-Seq Illumina” protocol on the IP-Star® is using the standard “NEBNext® ChIP-Seq Library Prep for Illumina” kit and reagents from NEB®. This kit... -
Download MicroPlex Library Preparation kit™ v2 - IP-Star Compact
The “MicroPlex Library Preparation” protocol on the IP-Star® is using the standard “MicroPlex Library Preparation™ kit” and reagents from Diagenode. The MicroPlex ... -
Download TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a nat...