ZEB1 polyclonal antibody - Classic

Catalog Number
25 μl
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Polyclonal antibody raised in rabbit against ZEB1 (zinc finger E-box binding homeobox 1), using a recombinant protein.

Concentration0.1 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 3

* Optimal dilutions/concentrations should be determined by the researcher.

  • Validation Data

    ChIP results

    Figure 1. ZEB1 ChIP results
    ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ZEB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.

    IP results

    Figure 2. IP results
    ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 μg of ZEB1 antibody (Cat. No. C15410259). The immunoprecipitated ZEB1 protein was detected by western blot with the ZEB1 antibody diluted 1:1,000.

    Western blot

    Figure 3. Western blot

    Whole cell extracts (30 μg) from 293T, A431, HeLa and HepG2 cells were analyzed by WB using the Diagenode ZEB1 antibody (C15410259) diluted 1:1000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.


    Figure 4. IF
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using the Diagenode ZEB1 antibody (Cat. No. C15410259) at a 1:500 dilution (green). The cytoskeleton was stained with phalloidin (red).

  • Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet ZEB1 C15410259 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ZEB1 polyclonal antibody - Classic (Diagenode Cat# C15410259-25 Lot# 42690). Click here to copy to clipboard.

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