The True MicroChIP-seq kit combined with the MicroPlex Library Preparation kitprovides a robust ChIP-seq protocol suitable for the investigation of histone modifications within chromatin from as few as 10 000 cells, including FACS sorted cells. The True MicroChIP-seq & MicroPlex Library Preparation™ Package contains everything you need for start-to-finish ChIP-seq including all validated buffers and reagents for chromatin shearing, immunoprecipitation, DNA purification and library preparation for exceptional ChIP-seq results. In addition, positive control antibodies and negative control PCR primers are included for your convenience and assurance of result sensitivity and specificity.
The True MicroChIP-seq& MicroPlex Library Preparation™ Package offers unique benefits:
An optimized chromatin preparation protocol compatible with low number of cells (10.000) in combination with the Bioruptor™ shearing device
Most complete kit available (covers all steps and includes control antibodies and primers)
Magnetic beads make ChIP easy, fast, and more reproducible
MicroChIP DiaPure columns (included in the kit) enable the maximum recovery of immunoprecipitation DNA suitable for any downstream application