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Figure 1. TRRAP ChIP results ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or TRRAP antibody. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.
Figure 2. TRRAP IP results TRRAP antibody immunoprecipitates TRRAP protein in IP experiments. IP samples: 293T whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of TRRAP protein by 4 μg TRRAP antibody (Cat. No. C15410257) 5 % SDS-PAGE The immunoprecipitated TRRAP protein was detected by TRRAP antibody (C15410257) diluted 1:500.
Figure 3. TRRAP IF results TRRAP antibody detects TRRAP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRRAP protein stained by TRRAP antibody (Cat. No. C15410257) diluted 1:1,000. Blue: Hoechst 33342 staining.
Figure 4. TRRAP Western blot results TRRAP antibody detects TRRAP protein by western blot analysis. A. 30 μg Jurkat whole cell extract B. 30 μg Raji whole cell extract C. 30 μg NCI-H929 whole cell extract 5 % SDS-PAGE TRRAP antibody (Cat. No. C15410257) dilution: 1:500.
Figure 5. TRRAP western blot results TRRAP antibody detects TRRAP protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431 whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 5 % SDS-PAGE TRRAP antibody (Cat. No. C15410257) dilution: 1:500.
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