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Figure 1. THOC1 ChIP results ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or THOC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to calreticulin.
Figure 2. THOC1 IP results THOC1 immunoprecipitates THOC1 (p84) protein in IP experiments. IP Sample: HepG2 whole cell extract A: 30 μg whole cell extract of THOC1 protein expressing HepG2 cells B: Control with 3 μg of pre-immune rabbit IgG C: Immunoprecipitation of THOC1 by 3 μg of THOC1 antibody. The immunoprecipitated THOC1 protein was detected by western blot with the THOC1 antibody diluted 1:1,000.
Figure 3. THOC1 IF results THOC1 antibody detects THOC1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: THOC1 protein stained by THOC1 antibody diluted 1:500. Blue: Hoechst 33342 staining.
Figure 4. THOC1 IHC results Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using THOC1 antibody at a 1:500 dilution.
Figure 5. THOC1 Western blot results Sample: 30 μg of A549 whole cell lysate 7.5% SDS PAGE THOC1 antibody diluted 1:1,000
IF Immunofluorescence:
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Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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