Polyclonal antibody raised in rabbit against TFIIF (general transcription factor IIF, polypeptide 1, 74kDa), using a recombinant protein.
| Lot | 40723 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3, 4 |
Figure 1. TFIIF ChIP results
ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
Figure 2. TFIIF IP results
TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of TFIIF protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C: Immunoprecipitation of TFIIF with 2.5 μg of TFIIF antibody (C15410256) The immunoprecipitated TFIIF protein was detected by western blot with the TFIIF antibody diluted 1:1,000.
Figure 3. Western blot
Sample: 30 μg of whole cell lysate A: 293T B: A431 C: HeLa 7.5% SDS PAGE TFIIF antibody dilution 1:3,000.
Figure 4. Western blot
Sample: 30 μg of NIH-3T3 whole cell lysate 7.5% SDS PAGE TFIIF antibody dilution 1:3,000
 How to properly cite this product in your workDiagenode strongly recommends using this: TFIIF polyclonal antibody - Classic (Diagenode Cat# C15410256-100 Lot# 40723). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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