Figure 1. ChIP results obtained with the Diagenode antibody directed against GTF2E2
ChIP assays were performed using HeLa cells, the Diagenode antibody against GTF2E2 (Cat. No. C15410264) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP- seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).