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TAL1 Antibody - ChIP-seq Grade (sample size)

Catalog Number
Format
Price
C15200012-10
10 µg
$95.00
  Bulk order
Other format



Monoclonal antibody raised in mouse against human TAL1 (T-Cell Acute Lymphocytic Leukemia 1), using a recombinant protein. 

Lot001
Concentration1 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityAffinity purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2-5 μg/ChIP Fig 1, 2
IF 1:1,000 Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation data

    TAL1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against TAL1
    ChIP was performed using K562 cells, the Diagenode monoclonal antibody against TAL1 (Cat. No. C15200012) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the RUNX1 and RUNX1T1 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    TAL1 Antibody ChIP-seq Grade

    TAL1 Antibody for ChIP-seq

    TAL1 Antibody for ChIP-seq assay

    TAL1 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TAL1
    ChIP was performed with 5 μg of the Diagenode antibody against TAL1 (Cat. No. C15200012) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2.5 mb region of chromosome 1 (figure 2A and B) and in two regions surrounding the RUNX1and RUNX1T1 positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.

    TAL1 Antibody validated in Immunofluorescence

    Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against TAL1
    K562 cells were stained with the Diagenode antibody against TAL1 (cat. No. C15200012) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the TAL1 antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.

  • Target Description

    TAL1 (UniProt/Swiss-Prot entry P17542) is a transcription factor involved in hemopoietic differentiation It functions as a positive regulator of erythroid differentiation. TAL1 is implicated in the genesis of hemopoietic malignancies such as t-cell leukemia and cd3epsilon deficiency.

  •  Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
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  •  Documents
    TAL1 monoclonal antibody DATASHEET
    Datasheet of the TAL1 monoclonal antibody
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TAL1 Antibody - ChIP-seq Grade (sample size) (Diagenode Cat# C15200012-10 Lot# 001). Click here to copy to clipboard.

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