Alternative names: CHET9, JJAZ1
Polyclonal antibody raised in rabbit against the N-terminus of recombinant human Suz12 protein (Suppressor of Zeste 12 protein homolog).
| Lot | 001 |
|---|---|
| Concentration | 2.0 µg/µl |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Protein G purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 10 μg/ChIP | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 μg per IP.
Figure 1. ChIP using the Diagenode antibody directed against Suz12
ChIP was performed using 600 μg of chromatin from mouse ES cells and 10 μg of the Diagenode antibody against Suz12 (Cat. No. pAb-029-050). IgG (10 μg) was used as a negative IP control. The IP’d DNA was analysed by QPCR with specific primer sets for different positive and negative genes. Figure 1 shows the results expressed as a % of input (the relative abundance of target DNA in the immunoprecipitates compared to the input DNA). Data provided by A. Helness, Epigenetics and Development Group, Faculty of Medicine Imperial College London, UK.
Figure 2. Western blot analysis using the Diagenode antibody directed against Suz12
HeLa nuclear extracts (HeLa NE, 20 μg) were analysed by Western blot using the Diagenode antibody directed against Suz12 (Cat. No. pAb-029-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (lane 2, expected size: 83 kDa). A molecular weight marker is shown in lane 1.
 How to properly cite this product in your workDiagenode strongly recommends using this: Suz12 polyclonal antibody - Classic (Diagenode Cat# C15410029 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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