SMAD3 polyclonal antibody - Classic

Catalog Number
25 μl
  Bulk order
Other format

Polyclonal antibody raised in rabbit against SMAD3 (SMAD family member 3), using a recombinant protein.

Concentration0.78 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
IHC 1:500 Fig 5
Immunoprecipitation 1:500 - 1:1,000 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data


    Figure 1. ChIP
    SMAD3 antibody immunoprecipitates SMAD3 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD3 antibody (C15410252) The precipitated DNA was detected by PCR with primer set targeting to SMAD7 promoter.


    Figure 2. Immunoprecipitation
    SMAD3 antibody immunoprecipitates SMAD3 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell extract A. 50 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SMAD3 protein by 2 μg of SMAD3 antibody (C15410252). The immunoprecipitated SMAD3 protein was detected by western blot with the SMAD3 antibody (C15410252) diluted 1:1,000.


    Figure 3. Immunofluorescence
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using SMAD3 (C15410252) antibody at a 1:200 dilution.

    Western Blot

    Figure 4. Western Blot
    Sample: 30 μg of Jurkat whole cell lysate 7.5% SDS PAGE C15410252 diluted 1:5,000


    Figure 5. Immunohistochemistry
    SMAD3 antibody detects SMAD3 protein at cytosol and nucleus on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded gastric carcinoma. SMAD3 antibody (C15410252) dilution: 1:500.

  •  Applications
    Enzyme-linked immunosorbent assay. Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunohistochemistry Read more
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet SMAD3 C15410252 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMAD3 polyclonal antibody - Classic (Diagenode Cat# C15410252-25 Lot# 40044). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  Related products


  • Genome instability: when RNA meets chromatin
    Sep 16-Jun 20, 2019
  • Japanese Biochemical Society
    Sep 18-Sep 20, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    Atlanta, Georgia
    Sep 19, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    RTP, North Carolina
    Sep 20, 2019
 See all events

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics