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<p><small><strong>Figure 1. Immunofluorescence using the Diagenode monoclonal antibody directed against psU</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against psU (cat. No. C15200236). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the psU antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 2. Immunohistochemistry using the Diagenode monoclonal antibody directed against psU</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against psU (cat. No. C15200236) diluted 1:1,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1534',
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(int) 5 => array(
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'countries' => 'ES',
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'created' => '2021-08-30 16:03:46',
'ProductsSafetySheet' => array(
[maximum depth reached]
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(int) 6 => array(
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'name' => 'Pseudouridine antibody SDS DE de',
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'url' => 'files/SDS/Pseudouridine/SDS-C15200236-Pseudouridine_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2021-08-30 16:03:46',
'created' => '2021-08-30 16:03:46',
'ProductsSafetySheet' => array(
[maximum depth reached]
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(int) 7 => array(
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'countries' => 'JP',
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'created' => '2021-08-30 16:03:46',
'ProductsSafetySheet' => array(
[maximum depth reached]
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)
)
)
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(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '32',
'position' => '10',
'parent_id' => '40',
'name' => 'IHC',
'description' => '<p>Immunohistochemistry</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunohistochemistry',
'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications',
'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:53',
'created' => '2015-07-08 13:49:03',
'ProductsApplication' => array(
'id' => '4887',
'product_id' => '2965',
'application_id' => '32'
)
)
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(int) 0 => 'immunohistochemistry'
)
$applications = array(
'id' => '32',
'position' => '10',
'parent_id' => '40',
'name' => 'IHC',
'description' => '<p>Immunohistochemistry</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunohistochemistry',
'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications',
'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:53',
'created' => '2015-07-08 13:49:03',
'locale' => 'eng'
)
$description = '<p>Immunohistochemistry</p>'
$name = 'IHC'
$document = array(
'id' => '997',
'name' => 'Pseudouridine monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to KLH.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/pseudouridine-monoclonal-antibody-100-ug.pdf',
'slug' => 'pseudouridine-monoclonal-antibody-datasheet',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-03-19 11:20:32',
'created' => '2018-03-19 11:20:32',
'ProductsDocument' => array(
'id' => '2586',
'product_id' => '2965',
'document_id' => '997'
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)
$sds = array(
'id' => '1535',
'name' => 'Pseudouridine antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Pseudouridine/SDS-C15200236-Pseudouridine_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-08-30 16:03:46',
'created' => '2021-08-30 16:03:46',
'ProductsSafetySheet' => array(
'id' => '2686',
'product_id' => '2965',
'safety_sheet_id' => '1535'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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