Other names: pSU
Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to KLH.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse, other (wide range): positive. |
| Type | Monoclonal |
| Purity | Protein G purified monoclonal antibody. |
| Host | Mouse |
| Storage Conditions | Store at -20°C. |
| Storage Buffer | PBS containing 50% glycerol, does not contain a preservative. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| IF | 1:1,000 | Fig 1 |
| IHC | 1:1,000 | Fig 2 |
Figure 1. Immunofluorescence using the Diagenode monoclonal antibody directed against psU
HeLa cells were stained with the Diagenode monoclonal antibody against psU (cat. No. C15200236). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the psU antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Figure 2. Immunohistochemistry using the Diagenode monoclonal antibody directed against psU
Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against psU (cat. No. C15200236) diluted 1:1,000 (brown). The slides were counterstained with Hematoxylin (blue).
 How to properly cite this product in your workDiagenode strongly recommends using this: Pseudouridine monoclonal antibody (Diagenode Cat# C15200236 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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