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Polyclonal antibody raised in rabbit against human PPARG (peroxisome proliferator-activated receptor gamma), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.
Affinity purified polyclonal antibody
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PBS containing 0.05% azide and 0.05% ProClin 300.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Fig 2, Ref 1
* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against PPARG ChIP was performed on macrophages derived from mouse bone marrow using the Diagenode antibody against PPARG (cat. No. CS-133-050) and optimized PCR primer sets for qPCR. Sheared chromatin from 1 million cells and 1 μg of PPARg antibody were used per ChIP experiment. IgG was used as a negative IP control. Figure 1A: recovery, expressed as the % of input, of the PDK4 PPAR response element (RE). Figure 1B: recovery of the FABP4 Adipo PPAR RE in cells treated with RSG, a very strong activating ligand of PPARG, and in untreated cells.
Figure 2. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PPARG (cat. No. CS-133-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:70,250
Figure 3. Western blot analysis using the Diagenode antibody directed against PPARG 293T cells were transfected with pNTAP-PPARG and 20 μg of protein extract was analysed by Western blot using the Diagenode antibody against PPARG (cat. No. CS-133-100). The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Figure 2 shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (in kDa) is shown on the left.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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