PKNOX1 polyclonal antibody - Classic

Catalog Number
25 μl
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Polyclonal antibody raised in rabbit against PKNOX1 (PBX/knotted 1 homeobox 1), using a recombinant protein.

Concentration1 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
Immunofluorescence 1:100 - 1:1,000 Fig 1
Immunoprecipitation 1:100 - 1:1,000 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 3
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    IF results

    Figure 1. PKNOX1 IF results
    PKNOX1 antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKNOX1 protein stained by PKNOX1 antibody diluted 1:1,000. Blue: Hoechst 33342 staining.

    Immunoprecipitation results

    Figure 2. PKNOX1 Immunoprecipitation results
    PKNOX1 antibody immunoprecipitates PKNOX1 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 protein by 2 μg of PKNOX1 antibody The immunoprecipitated PKNOX1 protein was detected by western blot with the PKNOX1 antibody diluted 1:1,000.

    Western blot results

    Figure 3. PKNOX1 Western blot results
    Sample: 30 μg of whole cell lysate A: HepG2 B: HepG2 nucleus 10% SDS PAGE PKNOX1 antibody diluted 1:2,000.

  •  Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    Datasheet PKNOX1 C15410267 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Publications

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