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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p>Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.</p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against PCBP1 (Cat. No. C15410359) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the PCBP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the PCBP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PCBP1</strong><br />Whole cell extracts from 293T, K562, HeLa, Jurkat, MCF7, NIH3T3, WR19L and Rat1 cells (lanes 1 to 8, respectively) were analysed by Western blot using the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against PCBP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode antibody against PCBP1 (<span>Cat</span>. No. C15410359, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PCBP1 protein was subsequently detected by western blot with the PCBP1 antibody as described above. The input is shown in lane 1.</small></p>
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'created' => '2020-06-08 16:47:46',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '233',
'name' => 'PCBP1 antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/PCBP1/SDS-C15410359-PCBP1_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:48:52',
'created' => '2020-06-08 16:46:54',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '237',
'name' => 'PCBP1 antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/PCBP1/SDS-C15410359-PCBP1_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2020-06-08 16:50:18',
'created' => '2020-06-08 16:50:18',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '236',
'name' => 'PCBP1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/PCBP1/SDS-C15410359-PCBP1_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:49:47',
'created' => '2020-06-08 16:49:47',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'ProductsApplication' => array(
'id' => '4948',
'product_id' => '2986',
'application_id' => '30'
)
)
$slugs = array(
(int) 0 => 'immunoprecipitation'
)
$applications = array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'locale' => 'eng'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
'id' => '1011',
'name' => 'PCBP1 polyclonal antibody datasheet',
'description' => '<p>Polyclonal antibody raised in rabbit against human PCBP1 (Poly(RC) Binding Protein 1), using a KLH-conjugated synthetic peptide from the central region of the protein.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => '../files/products/antibodies/pcbp1-polyclonal-antibody-100-ug.pdf',
'slug' => 'pcbp1-polyclonal-antibody-100-ug-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-07-11 15:48:14',
'created' => '2018-07-11 15:48:14',
'ProductsDocument' => array(
'id' => '2650',
'product_id' => '2986',
'document_id' => '1011'
)
)
$sds = array(
'id' => '236',
'name' => 'PCBP1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/PCBP1/SDS-C15410359-PCBP1_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:49:47',
'created' => '2020-06-08 16:49:47',
'ProductsSafetySheet' => array(
'id' => '459',
'product_id' => '2986',
'safety_sheet_id' => '236'
)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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