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Figure 1 Western blot analysis using the Diagenode antibody directed against p53K372me1 Western blot was performed on 100 μg Colon carcinoma whole cell extract (HCT116 WCE) using the Diagenode antibody against p53K372me1 (Cat. No. pAb-048-050), diluted 1:200 in TBS-Tween containing 5% skimmed milk. The size and location of the protein of interest are indicated (Figure 1, kindly provided by Liu Xiangyu, Health Science Center, Peking University, China).
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more