Diagenode

NRF2 polyclonal antibody

Antibody ChIP icon
Catalog Number
Format
Price
C15410242-100
100 μl
$335.00
  Bulk order
Other format

Other names: NFE2L2, HEBP1, TFIIE-B, FE

Polyclonal antibody raised in rabbit against NRF2 (NF-E2-Related Factor 2), using a recombinant protein.

Lot42347
Concentration0.58 μg/μl
Species reactivityHuman, rat
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 20% glycerol and 0.025% ProClin 300.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μg/ChIP Fig 1
Immunofluorescence 1:500 Fig 2
Immunohistochemistry 1:500 Fig 3
Western Blotting 1:1,000 Fig 4, 5
Immunoprecipitation 5 μg/IP Fig 6

* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 µg per IP.

  • Validation Data

    ChIP results

    Figure 1. ChIP results obtained with the Diagenode antibody directed against NRF2
    ChIP was performed on HeLa cells with 5 µg of the Diagenode antibody against NRF2 (Cat. No. C15410242). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the GCLC gene. Figure 1 shows the fold enrichment over the IgG negative control.

    IF results

    Figure 2. Immunofluorescence using the Diagenode antibody directed against NRF2
    HeLa cells were stained with the Diagenode antibody against NRF2 (Cat. No. C15410242). Cells were fixed with 4% formaldehyde for 15 min at RT and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the NRF2 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Phalloidin, a cytoskeleton marker.

    IHC results

    Figure 3. Immunohistochemistry using the Diagenode antibody directed against NRF2
    Paraffin-embedded Cal27 xenograft was analysed by immunohistochemical analysis, using the Diagenode antibody against NRF2 (Cat. No. C15410242) diluted 1:500.

    Western blot results

    Figure 4. Western blot analysis using the Diagenode antibody directed against NRF2
    Whole cell extracts from 293T cells (30 µg) transfected with a 3xFlag-tagged NRF2 expression vector (lane 2) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against NRF2 (Cat. No. C15410242) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Western blot results

    Figure 5. Western blot analysis using the Diagenode antibody directed against NRF2
    Nuclear extracts from MDA-MB-231 cells (30 µg) treated with 30 µM tBHQ for 4 hours (lane 2) or untreated control cells (lane 1) were analysed by western blot using the Diagenode antibody against NRF2 (cat. No. C15410242) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunoprecipitation results

    Figure 6. Immunoprecipitation using the Diagenode antibody directed against NRF2
    Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 5 µg of the Diagenode antibody against NRF2 (Cat. No. C15410242) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated NRF22 protein was detected by western blot with the NRF2 antibody diluted 1:1,000.

  •  Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet NRF2 C15410242 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: NRF2 polyclonal antibody (Diagenode Cat# C15410242-100 Lot# 42347). Click here to copy to clipboard.

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