Micrococcal Nuclease for Chromatin Assembly (500U/ml)

Catalog Number
1 ml

Micrococcal nuclease or MNase is a 16.9 kDa endonuclease derived from Staphylococcus aureus. It is purified from an E. coli strain expressing an N-terminal 6XHIS tagged micrococcal nuclease.

  • Nuclease description

    Purified protein exhibit an strong endonuclease activity against single-stranded, double-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. The rate of cleavage is 30 times greater at the 5’ side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3’-phosphates. MNase is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

  • Quality control

    Figure 1.
    SDS page of the micrococcal MNase. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Figure 2.
    MNase activity assay. 0.5 μg plasmid DNA was digested with the Diagenode MNase (concentration 5U/ml) in a buffer containing 20 mM Tris-HCl Ph 7.6, 3 mM CaCl2 and 0.01% BSA and analyzed by agarose gel eletrophoresis. The digestion was carried out for 0.5, 1, 2 and 4 minutes (lane 2, 3, 4 and 5, repectively). An undigested control is shown in lane 1.

  •  Documents
    C06070001 micrococcal nuclease DATASHEET
    C06070001 micrococcal nuclease tds
  •  Publications

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