Diagenode

Micrococcal Nuclease for Chromatin Assembly (500U/ml)

default alt
Catalog Number
Format
Price
C06070001
0.25 ml
$170.00

Micrococcal nuclease or MNase is a 16.9 kDa endonuclease derived from Staphylococcus aureus. It is purified from an E. coli strain expressing an N-terminal 6XHIS tagged micrococcal nuclease.

  • Nuclease description

    Purified protein exhibit an strong endonuclease activity against single-stranded, double-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. The rate of cleavage is 30 times greater at the 5’ side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3’-phosphates. MNase is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Micrococcal Nuclease for Chromatin Assembly (500U/ml) (Diagenode Cat# C06070001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  • Related products

Events

  • "Molecular Biosystems" Conference on Eukaryotic Gene Regulation and Functional Genomics
    Puerto Varas, Región de Los Lagos, Chile
    Sep 23-Sep 26, 2017
  • The 76th Annual Meeting of the Japanese Cancer Association
    Tokyo, Japan
    Sep 28-Sep 30, 2017
 See all events

Twitter feed

News

 See all news