Diagenode

N6-methyladenosine (m6A) polyclonal antibody

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410208-50
50 μg
$305.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.

LotA2125-0010
Concentration1.2 µg/µl
Species reactivityHuman, mouse, other (wide range)
TypePolyclonal
PurityProtein G purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide and 0.05% ProClin 300.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP* 2 µg per IP Fig 1
Dot Blotting 1:400 Fig 2

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.

  • Validation Data

    RIP

    Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A
    RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Dot blot

    Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A
    To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.

  •  Applications
    DB
    Dot blotting Read more
    RIP
    RIP Read more
  •  Documents
    Datasheet m6A C15410208 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: N6-methyladenosine (m6A) polyclonal antibody (Diagenode Cat# C15410208-50 Lot# A2125-0010). Click here to copy to clipboard.

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