Diagenode

LowCell# ChIP kit protein G

Product image
Catalog Number
Format
Price
C01010073
(kch-maglow-G48)
48 rxns
$960.00
Other format

The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 1000 - 10 000 cells per IP. Ideal for Stem cells, embryos and biopsies.

  • Characteristics
    • Use from 1,000 - 100,000 cells per IP
    • Improved handling and reproducibility by the use of magnetic beads and special Magnetic rack (0.2 ml tubes)
    • Optimized purification and de-crosslinking step
    • One day from cell collection to results

    The LowCell# ChIP kit has been validated using chromatin sheared by sonication using the Bioruptor®. The Kit includes a DNA purifying slurry for an extra-fast method to purify your IP’d material (for qPCR analysis). Alternatively, for other applications, e.g. sequencing, linear amplification or microarray, magnetic DNA purification (IPure) can be performed alternatively.

  • Protocols
    ChIP from plant material with the LowCell# ChIP Kit
    As preparation of plant material for Chromatin Immunoprecipiation differs essentially from prepar...
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    ChIP from tissues with the LowCell# ChIP kit
    In theory, you could use 30 mg of tissue per antibody per ChIP. You will start by optimizing cond...
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  • Applications
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  • Documents
    LowCell# ChIP kit MANUAL
    Diagenode provides kits with optimized reagents and simplified protocols for ChIP includi...
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    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: LowCell# ChIP kit protein G (Diagenode Cat# C01010073). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    5-hydroxymethylcytosine accumulation in postmitotic neurons results in functional demethylation of expressed genes
    Mellén M. et al.
    5-hydroxymethylcytosine (5hmC) occurs at maximal levels in postmitotic neurons, where its accumulation is cell-specific and correlated with gene expression. Here we demonstrate that the distribution of 5hmC in CG and non-CG dinucleotides is distinct and that it reflects the binding specificity and genome occupancy o...

    Epigenome profiling and editing of neocortical progenitor cells during development
    Albert M. et al.
    The generation of neocortical neurons from neural progenitor cells (NPCs) is primarily controlled by transcription factors binding to DNA in the context of chromatin. To understand the complex layer of regulation that orchestrates different NPC types from the same DNA sequence, epigenome maps with cell type resoluti...

    Vitamin C induces specific demethylation of H3K9me2 in mouse embryonic stem cells via Kdm3a/b
    Kevin T. Ebata, Kathryn Mesh, Shichong Liu, Misha Bilenky, Alexander Fekete, Michael G. Acker, Martin Hirst, Benjamin A. Garcia and Miguel Ramalho-Santos
    Background Histone methylation patterns regulate gene expression and are highly dynamic during development. The erasure of histone methylation is carried out by histone demethylase enzymes. We had previously shown that vitamin C enhances the activity of Tet enzymes in embryonic stem (ES) cells, leading to DNA...

    A chronic low dose of Δ9-tetrahydrocannabinol (THC) restores cognitive function in old mice
    Bilkei-Gorzo A. et al.
    The balance between detrimental, pro-aging, often stochastic processes and counteracting homeostatic mechanisms largely determines the progression of aging. There is substantial evidence suggesting that the endocannabinoid system (ECS) is part of the latter system because it modulates the physiological processes und...

    The Role of Angiotensin Converting Enzyme 1 within the median preoptic nucleus following chronic intermittent hypoxia
    Faulk K. et al.
    Sustained hypertension is an important consequence of obstructive sleep apnea. An animal model of the hypoxemia associated with sleep apnea, chronic intermittent hypoxia (CIH), produces increased sympathetic nerve activity (SNA) and sustained increases in blood pressure. Many mechanisms have been implicated in the h...

    Polycomb repressive complex 2 (PRC2) silences genes responsible for neurodegeneration
    von Schimmelmann M et al.
    Normal brain function depends on the interaction between highly specialized neurons that operate within anatomically and functionally distinct brain regions. Neuronal specification is driven by transcriptional programs that are established during early neuronal development and remain in place in the adult brain. The...

    MEP50/PRMT5 Reduces Gene Expression by Histone Arginine Methylation and this Is Reversed by PKCδ/p38δ Signaling
    Saha K et al.
    Protein kinase C δ (PKCδ) and p38δ are key proteins in a cascade that stimulates keratinocyte differentiation. This cascade activates transcription of involucrin (hINV) and other genes associated with differentiation. Protein arginine methyltransferase 5 (PRMT5) is an arginine methyltransferase tha...

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Events

  • Epigenetic Mechanisms in Health and Disease 2017
    Barcelona, Spain
    Oct 25-Oct 26, 2017
  • 35ème Congrès de la Societé Française de Toxicologie Génétique
    Marseille, France
    Oct 26-Oct 27, 2017
  • 7th Australian Epigenetics Conference
    Brisbane, Australia
    Oct 29-Nov 1, 2017
  • Australian Genomic Technologies Association (AGTA 2017 Annual Conference)
    Hobart, Tasmania
    Oct 29-Nov 1, 2017
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