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Figure 1. KDM6A ChIP results ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.
Figure 2. KDM6A WB results KDM6A antibody detects KDM6A protein by western blot analysis. A. 30 μg U87-MG whole cell extract B. 30 μg SK-N-SH whole cell extract C. 30 µg IMR32 whole cell extract D. 30 µg SK-N-AS whole cell extract 5 % SDS-PAGE KDM6A antibody dilution: 1:500
Figure 3. KDM6A IHC results Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A antibody at a 1:250 dilution.
Figure 4. KDM6A IFA results Immunofluorescence analysis of paraformaldehyde-fixed MCF-7 cells, using KDM6A antibody at a 1:500 dilution. ￼
Figure 5. KDM6A IP results KDM6A antibody immunoprecipitates KDM6A protein in IP experiments. IP samples: SK-N-SH whole cell extract A. 30 μg SK-N-SH whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of KDM6A protein by 4 µg KDM6A antibody 5 % SDS-PAGE The immunoprecipitated KDM6A protein was detected by KDM6A antibody diluted 1:500. ￼
Figure 6. KDM6A IHC results KDM6A antibody detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. KDM6A antibody dilution: 1:1,000. ￼
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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