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JMJD6 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410318
50 μg /50 μl
$295.00
  Bulk order

Alternative names: TDSR, PTDSR1, PSR

Polyclonal antibody raised in rabbit against human JMJD6 (Jumonji Domain Containing 6), using a recombinant protein.

Lot001
Concentration1 µg/µl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μg/ChIP Fig 1
Western Blotting 1:500 Fig 2
Immunohistochemistry 1:200 Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD6
    ChIP assays were performed using HeLa cells, the Diagenode antibody against JMJD6 (Cat. No. C15410318) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the SPI1 and SPOCD1, used as positive controls, and for the HBB promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    WB

    Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD6
    Whole protein extracts from mouse heart tissue were analysed by Western blot using the Diagenode antibody against JMJD6 (Cat. No. C15410318). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunohistochemistry

    Figure 3. Immunohistochemistry using the Diagenode antibody directed against JMJD6
    Formalin fixed paraffin embedded mouse brain tissue was stained with the Diagenode antibody against JMJD6 (Cat. No. C15410318) diluted 1:200 followed by a peroxidase labelled goat anti-rabbit secondary antibody.

  • Target description

    JMJD6 (UniProt/Swiss-Prot entry Q6NYC1) can act both as a histone arginine demethylase and a lysyl-hydroxylase. As an arginine demethylase it is able to demethylate histone H3 at Arg2 (H3R2me) and histone H4 at Arg3 (H4R3me), thereby playing an important role in the histone code. JMJD6 can also catalyze the 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 thereby acting as a regulator of RNA splicing. It is required for differentiation of multiple organs during embryogenesis and is a key regulator of hematopoietic differentiation.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IHC
    Immunohistochemistry Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet JMJD6 polyclonal antibody - C15410318 DATASHEET
    Datasheet of JMJD6 polyclonal antibody    		 Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...    		 Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...    		 Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: JMJD6 polyclonal antibody - Classic (Diagenode Cat# C15410318 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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