JMJD6 polyclonal antibody - Classic

Lot	001
Concentration	1 µg/µl
Species reactivity	Human, mouse
Type	Polyclonal

Purity	Affinity purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP ^*	5 μg/ChIP	Fig 1
Western Blotting	1:500	Fig 2
Immunohistochemistry	1:200	Fig 3

ChIP

Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD6
ChIP assays were performed using HeLa cells, the Diagenode antibody against JMJD6 (Cat. No. C15410318) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the SPI1 and SPOCD1, used as positive controls, and for the HBB promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD6
Whole protein extracts from mouse heart tissue were analysed by Western blot using the Diagenode antibody against JMJD6 (Cat. No. C15410318). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Immunohistochemistry

Figure 3. Immunohistochemistry using the Diagenode antibody directed against JMJD6
Formalin fixed paraffin embedded mouse brain tissue was stained with the Diagenode antibody against JMJD6 (Cat. No. C15410318) diluted 1:200 followed by a peroxidase labelled goat anti-rabbit secondary antibody.

Target description

JMJD6 (UniProt/Swiss-Prot entry Q6NYC1) can act both as a histone arginine demethylase and a lysyl-hydroxylase. As an arginine demethylase it is able to demethylate histone H3 at Arg2 (H3R2me) and histone H4 at Arg3 (H4R3me), thereby playing an important role in the histone code. JMJD6 can also catalyze the 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 thereby acting as a regulator of RNA splicing. It is required for differentiation of multiple organs during embryogenesis and is a key regulator of hematopoietic differentiation.

Applications

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

IHC
Immunohistochemistry Read more

ChIP-qPCR (ab)
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Documents

Datasheet JMJD6 polyclonal antibody - C15410318 DATASHEET Datasheet of JMJD6 polyclonal antibody	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download