Infinium Mouse Methylation & Hydroxymethylation Array Service

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The Infinium Mouse Methylation & Hydroxymethylation Array is a genome-wide DNA methylation analysis technique based on selective oxidation, bisulfite conversion and Illumina® technology. The technique allows to quantitively detect the true methylation (5mC) and total methylation (5mC + 5hmC) levels of over 285,000 methylation sites throughout the mouse genome at single nucleotide resolution. 

See our Infinium Mouse Methylation Array Service if only interested in the total methylation (5mC + 5hmC) level in the DNA sample

Comprehensive Coverage Across Gene Regions

  • Cost-effective solution with rapid turnaround time
  • Over 285,000 markers detected in mouse samples at single nucleotide resolution
  • Designed for common laboratory mouse strains
  • Balanced coverage of CpG islands, translation start sites, enhancers, imprinted loci, and other regions.
  • Differentially methylated site analysis using bioinformatic tools
  • End-to-end services include oxidative conversion, bisulfite conversion, array hybridization, and analysis

See our other DNA Methylation Profiling Services

  • Services Workflow

    End-to-end array 

    • Oxidative conversion
    • Bisulfite conversion
    • Whole genome amplification
    • Array hybridization
    • Single base extension  
    • Array scanner 
  • Bioinformatics Analysis




    Standard files provided:

    • Sample annotation
    • Variable annotation
    • Scanner output (IDAT files)

    Differential methylation analysis

    Identification of differentially methylated CpGs between sample groups.

    Files provided:

    • Report with summary of differential methylation analysis and plots
    • File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
    • File containing differential methylated regions (DMRs)

    Gene ontology terms analysis

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.

    Pathway analysis

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.

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