The Infinium Mouse Methylation & Hydroxymethylation Arrayis a genome-wide DNA methylation analysis technique based on selective oxidation, bisulfite conversion and Illumina® technology. The technique allows to quantitively detect the true methylation (5mC) and total methylation (5mC + 5hmC) levels of over 285,000 methylation sites throughout the mouse genome at single nucleotide resolution.
Raw data (un-normalized β values and detection p-values)
Scanner output
Differential methylation analysis
Identification of differentially methylated CpGs between sample groups.
Files provided:
Report with summary of differential methylation analysis and plots
File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
File containing differential methylated regions (DMRs)
Gene ontology terms analysis
Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.
Pathway analysis
Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.
Diagenode strongly recommends using this: Infinium Mouse Methylation & Hydroxymethylation Array Service (Diagenode Cat# G02160004). Click here to copy to clipboard.
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