Diagenode

IGF2BP1 monoclonal antibody

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Catalog Number
Format
Price
C15200237
100 µg
$270.00
  Bulk order

Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1

Monoclonal antibody raised in mouse against human IGF2BP1 (Insulin Like Growth Factor 2 MRNA Binding Protein 1), using the full length recombinant protein.

Lot001
Concentration1 µg/µl
Species reactivityHuman, mouse: positive. Other species: not tested.
TypeMonoclonal
PurityProtein A purified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C.
Storage BufferPBS containing 50% glycerol, does not contain a preservative.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP 15 µg per 107 cells Fig 1
Western Blotting 1:1,000 Fig 2
IP 5 µg per 5x106 cells Fig 3
  • Validation data

    RIP

    RIP

    Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against IGF2BP1
    Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode antibody against IGF2BP1 (cat. No. C15200237) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG2a control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    WB

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against IGF2BP1
    Whole cell extracts from HeLa, 293T, HL-60, NIH3T3 and MEF cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against IGF2BP1 (cat. No. C15200237) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IP

    Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against IGF2BP1
    Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP1 (cat. No. C15200237, lane 2). An equal amount of mouse IgG2a was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.

  • Applications
    RIP
    RIP Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  • Documents
    IGF2BP1 monoclonal antibody DATASHEET
    Monoclonal antibody raised in mouse against human IGF2BP1 (Insulin Like Growth Factor 2 MRNA Bind...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: IGF2BP1 monoclonal antibody (Diagenode Cat# C15200237 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

Events

  • The 12th Annual Meeting of the Japanese Society for Epigenetics
    Jun 24-Jun 25, 2018
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