Diagenode

HighCell# ChIP kit protein A

Product image
Catalog Number
Format
Price
C01010062
(kch-mahigh-A48)
48 rxns
$1,235.00
Other format

The Diagenode HighCell# ChIP kit protein A contains protein A-coated paramagnetic beads and negative IgG from rabbit. This kit is recommended to perform ChIP with rabbit polyclonal and monoclonal antibodies.

Perform ChIP with up to 10 million cells per IP. Recover more DNA and limit bias downstream applications.

  • Characteristics
    • Use from 1,000,000 - 10,000,000 cells per IP
    • Recover large amounts of DNA
    • Limit bias of possible downstream amplification steps (e.g. prior to next generation sequencing)
    • Ideal for ChIP of low abundant proteins
    • Improved handling and reproducibility due to magnetic beads and a uniquely designed magnetic rack (DiaMag1.5)
  • Applications
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  • Documents
    HighCell# ChIP kit MANUAL
    The Diagenode HighCell# ChIP kit protein A contains protein A-coated paramagnetic beads (...
    Download
    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HighCell# ChIP kit protein A (Diagenode Cat# C01010062). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    RNA-binding protein HuR enhances mineralocorticoid signaling in renal KC3AC1 cells under hypotonicity
    Lema I. et al.
    Mineralocorticoid receptor (MR) mediates the sodium-retaining action of aldosterone in the distal nephron. Herein, we decipher mechanisms by which hypotonicity increases MR expression in renal principal cells. We identify HuR (human antigen R), an mRNA-stabilizing protein, as an important posttranscriptional re...

    Stem cell-like transcriptional reprogramming mediates metastatic
    Mateo F. et al.
    Inhibitors of the mechanistic target of rapamycin (mTOR) are currently used to treat advanced metastatic breast cancer. However, whether an aggressive phenotype is sustained through adaptation or resistance to mTOR inhibition remains unknown. Here, complementary studies in human tumors, cancer models and cell lines ...

    Cistrome of the aldosterone-activated mineralocorticoid receptor in human renal cells
    Le Billan F. et al.
    Aldosterone exerts its effects mainly by activating the mineralocorticoid receptor (MR), a transcription factor that regulates gene expression through complex and dynamic interactions with coregulators and transcriptional machinery, leading to fine-tuned control of vectorial ionic transport in the distal nephron. To...

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