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HighCell# ChIP kit protein A

Catalog Number
Format
Price
C01010062
(kch-mahigh-A48)
48 rxns
$1,280.00
Other format

The Diagenode HighCell# ChIP kit protein A contains protein A-coated paramagnetic beads and negative IgG from rabbit. This kit is recommended to perform ChIP with rabbit polyclonal and monoclonal antibodies.

At present we highly recommend our new generation kits with optimized reagents and improved protocols:

For ChIP-qPCR: iDeal ChIP-qPCR Kit and iDeal ChIP FFPE Kit

For ChIP- seq: iDeal ChIP-seq Kit for Histones and iDeal ChIP-seq Kit for Transcription Factors

Check out our ChIP-grade and ChIP-seq grade antibodies.

 

  • Characteristics
    • Use from 1,000,000 - 10,000,000 cells per IP
    • Recover large amounts of DNA
    • Ideal for ChIP of low abundant proteins
    • Improved handling and reproducibility due to magnetic beads and a uniquely designed magnetic rack (DiaMag1.5)
  •  Documents
    HighCell# ChIP kit MANUAL
    The Diagenode HighCell# ChIP kit protein A contains protein A-coated paramagnetic beads (...
    Download
    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
  •  Safety sheets
    HighCell ChIP kit protein A SDS US en Download
    HighCell ChIP kit protein A SDS GB en Download
    HighCell ChIP kit protein A SDS FR fr Download
    HighCell ChIP kit protein A SDS DE de Download
    HighCell ChIP kit protein A SDS BE nl Download
    HighCell ChIP kit protein A SDS JP ja Download
    HighCell ChIP kit protein A SDS BE fr Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HighCell# ChIP kit protein A (Diagenode Cat# C01010062). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Seviteronel, a Novel CYP17 Lyase Inhibitor and Androgen Receptor Antagonist, Radiosensitizes AR-Positive Triple Negative Breast Cancer Cells
    Anna R. Michmerhuizen, Benjamin Chandler, Eric Olsen, Kari Wilder-Romans, Leah Moubadder, Meilan Liu, Andrea M. Pesch, Amanda Zhang, Cassandra Ritter, S. Tanner Ward, Alyssa Santola, Shyam Nyati, James M. Rae, Daniel Hayes, Felix Y. Feng, Daniel Spratt, D
    Increased rates of locoregional recurrence (LR) have been observed in triple negative breast cancer (TNBC) despite multimodality therapy, including radiation (RT). Recent data suggest inhibiting the androgen receptor (AR) may be an effective radiosensitizing strategy, and AR is expressed in 15–35% of TNBC tumo...

    A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells.
    Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D, Eggan K, Barrett LE
    CRISPR-Cas9-mediated gene interference (CRISPRi) and activation (CRISPRa) approaches hold promise for functional gene studies and genome-wide screens in human pluripotent stem cells (hPSCs). However, in contrast to CRISPR-Cas9 nuclease approaches, the efficiency of CRISPRi/a depends on continued expression of the de...

    ALK4 coordinates extracellular and intrinsic signals to regulate development of cortical somatostatin interneurons.
    Göngrich C, Krapacher FA, Munguba H, Fernández-Suárez D, Andersson A, Hjerling-Leffler J, Ibáñez CF
    Although the role of transcription factors in fate specification of cortical interneurons is well established, how these interact with extracellular signals to regulate interneuron development is poorly understood. Here we show that the activin receptor ALK4 is a key regulator of the specification of somatostatin in...

    UCP1 transrepression in Brown Fat in vivo and mineralocorticoid receptor anti-thermogenic effects.
    Kuhn E, Lamribet K, Viengchareun S, Le Menuet D, Fève B, Lombès M
    OBJECTIVES: The mineralocorticoid receptor (MR), a hormone-activated transcription factor, besides its role in controlling hydroelectrolytic homeostasis, exerts pro-adipogenic and anti-thermogenic effects, inhibiting mitochondrial-uncoupling protein UCP1 expression in brown adipocytes. The aim of this study was to g...

    HIV-2/SIV viral protein X counteracts HUSH repressor complex.
    Ghina Chougui, Soundasse Munir-Matloob, Roy Matkovic, Michaël M Martin, Marina Morel, Hichem Lahouassa, Marjorie Leduc, Bertha Cecilia Ramirez, Lucie Etienne   and Florence Margottin-Goguet
    To evade host immune defences, human immunodeficiency viruses 1 and 2 (HIV-1 and HIV-2) have evolved auxiliary proteins that target cell restriction factors. Viral protein X (Vpx) from the HIV-2/SIVsmm lineage enhances viral infection by antagonizing SAMHD1 (refs ), but this antagonism is not sufficient to explain a...

    NFE2-Related Transcription Factor 2 Coordinates Antioxidant Defense with Thyroglobulin Production and Iodination in the Thyroid Gland.
    Ziros PG, Habeos IG, Chartoumpekis DV, Ntalampyra E, Somm E, Renaud CO, Bongiovanni M, Trougakos IP, Yamamoto M, Kensler TW, Santisteban P, Carrasco N, Ris-Stalpers C, Amendola E, Liao XH, Rossich L, Thomasz L, Juvenal GJ, Refetoff S, Sykiotis GP
    BACKGROUND: The thyroid gland has a special relationship with oxidative stress. While generation of oxidative substances is part of normal iodide metabolism during thyroid hormone synthesis, the gland must also defend itself against excessive oxidation in order to maintain normal function. Antioxidant and detoxifica...

    T Cell Receptor-Regulated TGF-β Type I Receptor Expression Determines T Cell Quiescence and Activation.
    Tu E, Chia CPZ, Chen W, Zhang D, Park SA, Jin W, Wang D, Alegre ML, Zhang YE, Sun L, Chen W
    It is unclear how quiescence is enforced in naive T cells, but activation by foreign antigens and self-antigens is allowed, despite the presence of inhibitory signals. We showed that active transforming growth factor β (TGF-β) signaling was present in naive T cells, and T cell receptor (TCR)...

    HDAC1 and HDAC2 Modulate TGF-β Signaling during Endothelial-to-Hematopoietic Transition.
    Thambyrajah R, Fadlullah MZH, Proffitt M, Patel R, Cowley SM, Kouskoff V, Lacaud G
    The first hematopoietic stem and progenitor cells are generated during development from hemogenic endothelium (HE) through trans-differentiation. The molecular mechanisms underlying this endothelial-to-hematopoietic transition (EHT) remain poorly understood. Here, we explored the role of the epigenetic regulators HD...

    RNA-binding protein HuR enhances mineralocorticoid signaling in renal KC3AC1 cells under hypotonicity
    Lema I. et al.
    Mineralocorticoid receptor (MR) mediates the sodium-retaining action of aldosterone in the distal nephron. Herein, we decipher mechanisms by which hypotonicity increases MR expression in renal principal cells. We identify HuR (human antigen R), an mRNA-stabilizing protein, as an important posttranscriptional re...

    Stem cell-like transcriptional reprogramming mediates metastatic
    Mateo F. et al.
    Inhibitors of the mechanistic target of rapamycin (mTOR) are currently used to treat advanced metastatic breast cancer. However, whether an aggressive phenotype is sustained through adaptation or resistance to mTOR inhibition remains unknown. Here, complementary studies in human tumors, cancer models and cell lines ...

    Cistrome of the aldosterone-activated mineralocorticoid receptor in human renal cells
    Le Billan F. et al.
    Aldosterone exerts its effects mainly by activating the mineralocorticoid receptor (MR), a transcription factor that regulates gene expression through complex and dynamic interactions with coregulators and transcriptional machinery, leading to fine-tuned control of vectorial ionic transport in the distal nephron. To...

    The Integrase Cofactor LEDGF/p75 Associates with Iws1 and Spt6 for Postintegration Silencing of HIV-1 Gene Expression in Latently Infected Cells.
    Gérard A, Ségéral E, Naughtin M, Abdouni A, Charmeteau B, Cheynier R, Rain JC, Emiliani S
    The persistence of a latent reservoir containing transcriptionally silent, but replication-competent, integrated provirus is a serious challenge to HIV eradication. HIV integration is under the control of LEDGF/p75, the cellular cofactor of viral integrase. Investigating possible postintegration roles for LEDGF/p75,...

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