1ml frozen cell vial containing 1 x 106 cells
Cell Line validation
1ml frozen cell vial containing 1 x 106 cells. Expression of histone CENP-A is confirmed by immunodetection and confocal microscopy. Stable expression over multiple cell passages is confirmed. The doubling time for the cell line was approximately 20 h for the linear section of the growth curve.
Left panel corresponds to immunodetection of CENP-A with the anti-HA antibody.
Right panel shows the Dapi DNA staining.
Mycoplasma negative by DNA staining and direct culture methods.