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H3K27me2/3 monoclonal antibody - Classic

Catalog Number
50 µg/34 µl
  Bulk order

Monoclonal antibody raised in mouse against histone H3, di- or trimethylated at lysine 27 (H3K27me2/3), using a KLH-conjugated synthetic peptide.

Concentration1.5 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 1.5 μl Ref 1
Dot Blotting 1:300 Fig 1
Western Blotting 1:1,000 Fig 2
Immunofluorescence 1:100 Ref 1
  • Validation Data

    Dot Blot

    Figure 1. Cross reactivity of the Diagenode monoclonal antibody directed against H3K27me2/3
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode monoclonal antibody against H3K27me2/3 (cat# MAb-014-050) with peptides containing other modifications of histone H3. Other modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of lysines 9 and 36. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:300.

    Dot Blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody against H3K27me2/3
    Histone extracts from HeLa cells (HeLa HE, 20 μg) were analysed by Western blot using the Diagenode monoclonal antibody against H3K27me2/3 (cat# MAb-013-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right.

  •  Applications
    Dot blotting Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3K27me2-3 MAb-014-050 DATASHEET
    Datasheet description
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K27me2/3 monoclonal antibody - Classic (Diagenode Cat# C15200014 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Identification of Novel Molecular Markers of Human Th17 Cells.
    Sałkowska A, Karaś K, Karwaciak I, Walczak-Drzewiecka A, Krawczyk M, Sobalska-Kwapis M, Dastych J, Ratajewski M
    Th17 cells are important players in host defense against pathogens such as , , and . Th17 cell-mediated inflammation, under certain conditions in which balance in the immune system is disrupted, is the underlying pathogenic mechanism of certain autoimmune disorders, e.g., rheumatoid arthritis, Graves' disease, multi...

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