H3K27me2/3 monoclonal antibody - Classic

Antibody ChIP icon
Catalog Number
50 µg/34 µl
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Monoclonal antibody raised in mouse against histone H3, di- or trimethylated at lysine 27 (H3K27me2/3), using a KLH-conjugated synthetic peptide.

Concentration1.5 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 1.5 μl Ref 1
Dot Blotting 1:300 Fig 1
Western Blotting 1:1,000 Fig 2
Immunofluorescence 1:100 Ref 1
  • Validation Data

    Dot Blot

    Figure 1. Cross reactivity of the Diagenode monoclonal antibody directed against H3K27me2/3
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode monoclonal antibody against H3K27me2/3 (cat# MAb-014-050) with peptides containing other modifications of histone H3. Other modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of lysines 9 and 36. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:300.

    Dot Blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody against H3K27me2/3
    Histone extracts from HeLa cells (HeLa HE, 20 μg) were analysed by Western blot using the Diagenode monoclonal antibody against H3K27me2/3 (cat# MAb-013-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right.

  • Applications
    Dot blotting Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H3K27me2-3 MAb-014-050 DATASHEET
    Datasheet description
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K27me2/3 monoclonal antibody - Classic (Diagenode Cat# C15200014 Lot# 001). Click here to copy to clipboard.

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