Figure 1 ChIP results obtained with the Diagenode antibody directed against H2Bpan
ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2B (cat. No. C15410157) and optimized PCR primer sets for qPCR. ChIP was performed with the Auto Histone ChIP-seq” kit (cat. No. C01010022) on sheared chromatin from 1 million cells using the IP-Star automated system. A titration of the antibody consisting of 0.5, 1, 2 and 5 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoters if the active GAPDH and EIF4A2 genes, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis.