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Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against EZH2 ChIP assays were performed using 2.5 μg of the Diagenode monoclonal antibody against EZH2 (Cat. No. MAb-180-050) on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-/- cells, used as a negative control. IgG was used as negative IP control. Quantitative PCR was performed with primers specific for the HoxA10 gene and for the T gene which encodes the Brachyury transcription factor. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).