EZH2 Antibody

Catalog Number
50 μg/50 μl
  Bulk order
Other format

Alternative names: ENX-1, KMT6, EZH1

Monoclonal antibody raised in mouse against the central part of the human EZH2 protein (Enhancer of zeste homolog 2).

Concentration1.0 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2.5 μg/ChIP Fig 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    EZH2 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against EZH2
    ChIP assays were performed using 2.5 μg of the Diagenode monoclonal antibody against EZH2 (Cat. No. MAb-180-050) on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-/- cells, used as a negative control. IgG was used as negative IP control. Quantitative PCR was performed with primers specific for the HoxA10 gene and for the T gene which encodes the Brachyury transcription factor. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  • Target Description

    EZH2 (UniProt/Swiss-Prot entry Q15910) is a histone-lysine methyltransferase which methylates ‘Lys-9’ and ‘Lys-27’ of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and central nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer.

  •  Applications
  •  Documents
    Datasheet EZH2 MAb-180-050 DATASHEET
    Datasheet description
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
  •  Safety sheets
    EZH2 Antibody - ChIP Grade SDS GB en Download
    EZH2 Antibody - ChIP Grade SDS US en Download
    EZH2 Antibody - ChIP Grade SDS BE nl Download
    EZH2 Antibody - ChIP Grade SDS BE fr Download
    EZH2 Antibody - ChIP Grade SDS FR fr Download
    EZH2 Antibody - ChIP Grade SDS ES es Download
    EZH2 Antibody - ChIP Grade SDS DE de Download
    EZH2 Antibody - ChIP Grade SDS JP ja Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: EZH2 Antibody (Diagenode Cat# C15200180 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycleprogression and proliferation
    Samdal, H. et al.
    Long noncoding RNAs (lncRNAs) are involved in the regulation of cell cycle, although only a few have been functionally characterized. By combining RNA sequencing and ChIP sequencing of cell cycle synchronized HaCaT cells we have previously identified lncRNAs highly enriched for cell cycle functions. Based on a cycli...

    The specific alteration of histone methylation profiles by DZNep during early zebrafish development.
    Ostrup O, Reiner AH, Aleström P, Collas P
    Early embryo development constitutes a unique opportunity to study acquisition of epigenetic marks, including histone methylation. This study investigates the in vivo function and specificity of 3-deazaneplanocin A (DZNep), a promising anti-cancer drug that targets polycomb complex genes. One- to two-cell stage embr...

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