EZH2 monoclonal antibody - Classic

Catalog Number
50 μg/50 μl
  Bulk order

Alternative names: ENX-1, KMT6, EZH1

Monoclonal antibody raised in mouse against the central part of the human EZH2 protein (Enhancer of zeste homolog 2).

Concentration1.0 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2.5 μg/ChIP Fig 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data


    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against EZH2
    ChIP assays were performed using 2.5 μg of the Diagenode monoclonal antibody against EZH2 (Cat. No. MAb-180-050) on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-/- cells, used as a negative control. IgG was used as negative IP control. Quantitative PCR was performed with primers specific for the HoxA10 gene and for the T gene which encodes the Brachyury transcription factor. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  •  Applications
  •  Documents
    Datasheet EZH2 MAb-180-050 DATASHEET
    Datasheet description
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: EZH2 monoclonal antibody - Classic (Diagenode Cat# C15200180 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The specific alteration of histone methylation profiles by DZNep during early zebrafish development.
    Ostrup O, Reiner AH, Aleström P, Collas P
    Early embryo development constitutes a unique opportunity to study acquisition of epigenetic marks, including histone methylation. This study investigates the in vivo function and specificity of 3-deazaneplanocin A (DZNep), a promising anti-cancer drug that targets polycomb complex genes. One- to two-cell stage embr...

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