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EZH2 Antibody - ChIP Grade (sample size)

Catalog Number
Format
Price
C15200180-10
(MAb-180-050)
10 µg
$95.00
  Bulk order
Other format



Alternative names: ENX-1, KMT6, EZH1

Monoclonal antibody raised in mouse against the central part of the human EZH2 protein (Enhancer of zeste homolog 2).

Lot001
Concentration1.0 µg/µl
Species reactivityHuman
TypeMonoclonal
PurityProtein G purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2.5 μg/ChIP Fig 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    EZH2 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against EZH2
    ChIP assays were performed using 2.5 μg of the Diagenode monoclonal antibody against EZH2 (Cat. No. MAb-180-050) on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-/- cells, used as a negative control. IgG was used as negative IP control. Quantitative PCR was performed with primers specific for the HoxA10 gene and for the T gene which encodes the Brachyury transcription factor. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  • Target Description

    EZH2 (UniProt/Swiss-Prot entry Q15910) is a histone-lysine methyltransferase which methylates ‘Lys-9’ and ‘Lys-27’ of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and central nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer.

  •  Applications
  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Datasheet EZH2 MAb-180-050 DATASHEET
    Datasheet description
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: EZH2 Antibody - ChIP Grade (sample size) (Diagenode Cat# C15200180-10 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The specific alteration of histone methylation profiles by DZNep during early zebrafish development.
    Ostrup O, Reiner AH, Aleström P, Collas P
    Early embryo development constitutes a unique opportunity to study acquisition of epigenetic marks, including histone methylation. This study investigates the in vivo function and specificity of 3-deazaneplanocin A (DZNep), a promising anti-cancer drug that targets polycomb complex genes. One- to two-cell stage embr...

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