>>>   Click for Diagenode’s approach to COVID-19

E2F6 Antibody - ChIP-seq Grade

Catalog Number
50 μg
  Bulk order

Polyclonal antibody raised in rabbit against human E2F6 (E2F Transcription Factor 6), using a recombinant protein. 

Concentration1 µg/µl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 5 μg/ChIP Fig 1, 2
Western Blotting 1:500 Fig 3
Immunofluorescence 1:100 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.

  • Validation data

    E2F6 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against E2F6
    ChIP assays were performed using HeLa cells, the Diagenode antibody against E2F6 (Cat. No. C15410314) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the BRCA1, CBX5 and RBBP8 promoters, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    E2F6 Antibody ChIP-seq Grade

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against E2F6
    ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 5 μg of the Diagenode antibody against E2F6 (Cat. No. C15410314) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 400 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and CBX5 positive control genes, respectively (figure 2C and D).

    E2F6 Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against E2F6
    Whole cell extracts from Jurkat, HeLa and HepG2 cells (lane 1, 2 and 3, respectively) were analysed by Western blot using the Diagenode antibody against E2F6 (Cat. No. C15410314) diluted 1:500. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    E2F6 Antibody validated in Immunofluorescence

    Figure 4. Immunofluorescence using the Diagenode antibody directed against E2F6
    HeLa cells were stained with the Diagenode antibody against E2F6 (Cat. C15410314) diluted 1:100.

  • Target Description

    E2F6 (UniProt/Swiss-Prot entry O75461) is a member of the E2F family of transcription factors. It lacks the transcriptional activation and tumor suppressor protein association domains found in other family members but contains a modular suppression domain that functions as a transcriptional inhibitor. E2F6 plays a crucial role in the control of the cell cycle as it regulates a subset of E2F-dependent genes required for entry into the cell cycle but not for normal cell cycle progression. Overexpression of E2F6 delays the exit of cells from the S-phase.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet E2F6 polyclonal antibody - C15410314 DATASHEET
    Datasheet of E2F6 polyclonal antibody
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: E2F6 Antibody - ChIP-seq Grade (Diagenode Cat# C15410314 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  Related products

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics