Diagenode

CIRBP polyclonal antibody

Catalog Number
Format
Price
C15410360
100 µg
$315.00
  Bulk order

Other names: A18HNRNP, CIRP

Polyclonal antibody raised in rabbit against human CIRBP (Cold Inducible RNA Binding Protein), using a KLH-conjugated synthetic peptide from the N-terminus of the protein.

Lot001
Concentration1 μg/μl
Species reactivityHuman, rat: positive. Other species: not tested
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C.
Storage BufferPBS containing 50% glycerol, does not contain a preservative.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP 15 µg per 107 cells Fig 1
Western blotting 1:1,000 Fig 2
IP 5 µg per 5x106 cells Fig 3
  • Validation data

    ChIP

    RIP

    Figure 1. Immunoprecipitation using the Diagenode antibody directed against CIRBP
    Immunoprecipitation was performed on total RNA isolated from 10 million Jurkat cells using 15 µg of the Diagenode antibody against CIRBP (Cat. No. C15410360) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the CIRBP antibody (upper right). The lower figure shows the gel image for the negative IgG control, the CIRBP antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    WB

    Figure 2. Western blot analysis using the Diagenode antibody directed against CIRBP
    Whole cell extracts from HeLa, 293T, K562, Jurkat and Rat1 cells (lanes 1 to 5, respectively) were analysed by Western blot using the Diagenode antibody against CIRBP (Cat. No. C15410360) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IP

    Figure 3. Immunoprecipitation using the Diagenode antibody directed against CIRBP
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 5 µg of the Diagenode antibody against CIRBP (Cat. No. C15410360, lane 3). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CIRBP protein was subsequently detected by western blot with the CIRBP antibody as described above. The input is shown in lane 1.

  •  Applications
    RIP
    RIP Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  •  Documents
    CIRBP polyclonal antibody datasheet DATASHEET
    Polyclonal antibody raised in rabbit against human CIRBP (Cold Inducible RNA Binding Protein), us...
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  •  Publications

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