- Ultra-low inputs down to 50pg
- Starting material: fragmented single stranded DNA (ssDNA) or double stranded DNA (dsDNA)
- No primer dimers at the end of library preparation
- Ligation-free workflow
- Short, easy one-tube protocol
Diagenode’s [beta] DNA library preparation utilizes the innovative “Capture and Amplification by Tailing and Switching” (CATS), a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of single stranded DNA. CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching on the 5’- ends. The Diagenode kits generate ready-to-sequence libraries from DNA inputs as low as few picograms in just few hours. The CATS method also allows for accurate identification of circulating cell-free DNA or degraded DNA.