Diagenode’s [beta] DNA library preparation utilizes the innovative “Capture and Amplification by Tailing and Switching” (CATS), a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of single stranded DNA. CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching on the 5’- ends. The Diagenode kits generate ready-to-sequence libraries from DNA inputs as low as few picograms in just few hours. The CATS method also allows for accurate identification of circulating cell-free DNA or degraded DNA.
Excellent for single stranded DNA, starting with ultra-low inputs at 50pg
Perfect for challenging samples: cfDNA, bisulfite converted DNA, FFPE-derived DNA
Any type of fragmented DNA (fragmentase-treated or sheared)
Ligation-free workflow
No primer dimers at the end of library preparation
DNA/RNA library preparation
Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing
After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tail... Read more
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