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Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against BAP1 Figure 1A. Whole cell extracts from human NB4 cells (30 μg, lane 1) or mouse M1 cells (lane 2) were analysed by Western blot using the Diagenode antibody against BAP1 (cat. No. C15200212), diluted 1:1,000 in PBS containing 10% milk. The position of the protein of interest (expected MW 80 kDa) is indicated on the right; the marker (in kDa) is shown on the left. Figure 1B. Antibody titration on NB4 (1) and M1 (2) cells with the Diagenode BAP1 antibody.
Figure 2. Immunoprecipitation using the Diagenode monoclonal antibody directed against BAP1 IP was performed on 1 mg RIPA cell lysate from human NB4 cells (lane 1-3) or mouse M1 cells (lane 4-6) using the Diagenode antibody against BAP1 (cat. No. C15200212) diluted 1:400 (lane 3 and 6) or an IgG negative control (lane 2 and 5). The samples were analysed by Western blot analysis as described above. The input sample (30 μg RIPA lysate) was used as a positive control (lane 1 and 4).
Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against BAP1 HeLa cells were stained with the Diagenode antibody against BAP1 (Cat. No. C15200212) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the BAP1 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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