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BAP1 monoclonal antibody

Catalog Number
10 µg
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Alternative names: HUCEP-13, UCHL2, TPDS

Monoclonal antibody raised in mouse against human BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-Terminal Hydrolase)) using the full length recombinant protein.

Concentration1 µg/µl
Species reactivityHuman, mouse
PurityProtein G purified
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 - 1:2,000 Fig 1, 2
IP 1:400 Fig 2
IF 1:1,000 Fig 3

* Optimal dilution should be determined by the end-user.

  • Validation data

    Western Blot

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against BAP1
    Figure 1A. Whole cell extracts from human NB4 cells (30 μg, lane 1) or mouse M1 cells (lane 2) were analysed by Western blot using the Diagenode antibody against BAP1 (cat. No. C15200212), diluted 1:1,000 in PBS containing 10% milk. The position of the protein of interest (expected MW 80 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
    Figure 1B. Antibody titration on NB4 (1) and M1 (2) cells with the Diagenode BAP1 antibody.


    Figure 2. Immunoprecipitation using the Diagenode monoclonal antibody directed against BAP1
    IP was performed on 1 mg RIPA cell lysate from human NB4 cells (lane 1-3) or mouse M1 cells (lane 4-6) using the Diagenode antibody against BAP1 (cat. No. C15200212) diluted 1:400 (lane 3 and 6) or an IgG negative control (lane 2 and 5). The samples were analysed by Western blot analysis as described above. The input sample (30 μg RIPA lysate) was used as a positive control (lane 1 and 4).


    Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against BAP1
    HeLa cells were stained with the Diagenode antibody against BAP1 (Cat. No. C15200212) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the BAP1 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunoprecipitation Read more
  •  Documents
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Datasheet BAP1 C15200212 DATASHEET
    Datasheet description
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: BAP1 monoclonal antibody (Diagenode Cat# C15200212-10 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    PR-DUB maintains expression of critical genes through FOXK1/2 andASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1deubiquitination.
    Kolovos, P and Nishimura, K and Sankar, A and Sidoli, S and Cloos, PA andHelin, K and Christensen, J
    Polycomb group proteins are important for maintaining gene expression patterns and cell identity in metazoans. The mammalian Polycomb repressive de-ubiquitinase (PR-DUB) complexes catalyze removal of monoubiquitination on lysine 119 of histone H2A (H2AK119Ub1) through a multi-protein core comprised of BAP1, HCFC1, F...


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