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CATS Small RNA-seq Kit x12

Catalog Number
Format
Price
C05010044
12 rxns
Other format
DISCONTINUED

An updated version of this kit using D-Plex technology is available for superior performance.

Discover our D-Plex Small RNA-seq kit for Illumina and D-Plex Small DNBSEQ kit for MGI sequencing.

  • Characteristics
    • Ligation-free assay: increased efficiency and minimum bias
    • Low inputs: down to 10 picograms
    • Higher small RNA diversity
    • User-friendly: reduced hands-on time with fewer steps than competing methods
    • Libraries ready to sequence in only 5 hours
    • Retaining strand specificity of origin
  •  Testimonials

    Given the low input range of up to 1 ng of our cell free plasma derived small RNA samples, commercially available kits and various published protocols failed in generating a library suitable for NGS with attempts resulting in high concentrations of adaptor dimer or failed library amplifications.

    In light of this, we are more than happy to have tested the CATS small RNA-seq kit by Diagenode. Even with limited input material we got a rich library with absolutely no detectable adaptor dimer, as expected given it is a ligation free method. The kit performed superbly, in contrast to other commercially available kits or published protocols, with less hassle and with a greater ease of use.

    Highly recommended for anyone who is struggling with generating a library from low input and challenging samples.

     

    Jonatan Darr, PhD, Environmental Epigenetics Group, Institute of Experimental Genetics, Helmholtz Zentrum München, Germany
  •  Documents
    CATS Small RNA-seq kit MANUAL
    CATS Small RNA sequencing kit for Illumina
    Download
    Diagenode trimming tools for CATS library preparation [read1]
    Due to special mechanisms in creating CATS libraries such as template switching and artificial po...
    Download
    Diagenode trimming tools for CATS library preparation MANUAL
    This document describes the usage of the Diagenode trimming tools for CATS library preparation, w...
    Download
    Material Safety Data Sheet CATS small RNA-seq Kit DATASHEET
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CATS Small RNA-seq Kit x12 (Diagenode Cat# C05010044). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Repeat RNAs associate with replication forks and post-replicative DNA.
    Gylling HM, Gonzalez-Aguilera C, Smith MA, Kaczorowski DC, Groth A, Lund AH
    Non-coding RNA has a proven ability to direct and regulate chromatin modifications by acting as scaffolds between DNA and histone-modifying complexes. However, it is unknown if ncRNA plays any role in DNA replication and epigenome maintenance, including histone eviction and re-instalment of histone-modifications aft...

    Multisite Evaluation of Next-Generation Methods for Small RNA Quantification.
    Herbert ZT, Thimmapuram J, Xie S, Kershner JP, Kolling FW, Ringelberg CS, LeClerc A, Alekseyev YO, Fan J, Podnar JW, Stevenson HS, Sommerville G, Gupta S, Berkeley M, Koeman J, Perera A, Scott AR, Grenier JK, Malik J, Ashton JM, Pivarski KL, Wang X, Kuffe
    Small RNAs (smRNAs) are important regulators of many biologic processes and are now most frequently characterized using Illumina sequencing. However, although standard RNA sequencing library preparation has become routine in most sequencing facilities, smRNA sequencing library preparation has historically been chall...

    Circulating miRNA analysis for cancer diagnostics and therapy.
    Valihrach L, Androvic P, Kubista M
    Successful treatment of cancer depends on early diagnosis and effective monitoring of patients' response to therapy. Traditional tools based on tumor biopsies lack the sensitivity and specificity to capture cancer development in its early phases and are not applicable for continuous monitoring. To overcome these bar...

    The sncRNA Zoo: a repository for circulating small noncoding RNAs in animals.
    Fehlmann T, Backes C, Pirritano M, Laufer T, Galata V, Kern F, Kahraman M, Gasparoni G, Ludwig N, Lenhof HP, Gregersen HA, Francke R, Meese E, Simon M, Keller A
    The repertoire of small noncoding RNAs (sncRNAs), particularly miRNAs, in animals is considered to be evolutionarily conserved. Studies on sncRNAs are often largely based on homology-based information, relying on genomic sequence similarity and excluding actual expression data. To obtain information on sncRNA expres...

    MicroRNAs and Transplantation.
    Khan Z, Suthanthiran M, Muthukumar T
    miRNAs, ∼20 to 22 nucleotide single-stranded RNA species that play a pivotal role in the regulation of protein-coding genes, are emerging as robust biomarkers for assessing allograft status. Herein, the authors briefly review the biogenesis and function of the miRNAs and provide an overview of the tools to quant...

    NGS analysis of total small non coding RNAs from low input RNA from dried blood sampling
    Marcello Pirritano, Tobias Fehlmann, Thomas Laufer, Nicole Ludwig, Gilles Gasparoni, Yongping Li, Eckart Meese, Andreas Keller, and Martin Simon
    Circulating miRNAs are favored for biomarker candidates as they can reflect tissue specific miRNA dysregulation in disease contexts. Moreover, they have additional advantages that they can be monitored in a minimal invasive manner. Blood-borne miRNAs are therefore currently characterized to identify, describe and va...

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