5-hmC, 5-mC & cytosine DNA standard pack for hMeDIP

Catalog Number
40 rxns

The hydroxymethylated DNA standard is a linear dsDNA that has been produced by PCR using the Diagenode’s MethylTaq DNA polymerase and Diagenode’s hydroxymethyl dCTPs replacing normal cytosine nucleotides. The methylated and unmethylated standards have been produced using synthetic oligonucleotides. For the methylated standard, the normal cytosine nucleotides have been replaced by methylcytosine nucleotides. None of these standard show significant homology with human, mouse or rat genomic sequences.The sequences of these controls are different allowing for using all of them in the same tube.

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    DNA Methylation
    Complete solutions for DNA methylation studies Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data betwee... Read more
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    How to properly cite this product in your work

    Diagenode strongly recommends using this: 5-hmC, 5-mC & cytosine DNA standard pack for hMeDIP (Diagenode Cat# C02040011). Click here to copy to clipboard.

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    Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood.
    Kochmanski JJ, Marchlewicz EH, Cavalcante RG, Perera BPU, Sartor MA, Dolinoy DC
    BACKGROUND: Epigenetic machinery plays an important role in genomic imprinting, a developmental process that establishes parent-of-origin-specific monoallelic gene expression. Although a number of studies have investigated the role of 5-methylcytosine in imprinting control, the contribution of 5-hydroxymethylcytosin...

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