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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m3G</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 10 µg of the Diagenode monoclonal antibody against m3G (cat. No. C15200239) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 (left) shows the Bioanalyzer profile obtained with the input RNA (top), the m3G antibody (middle) and the negative control (bottom). The position of the small, 18s and 28s RNA in the input is indicated (1, 2 and 3, respectively). The right figure shows the gel image for the m3G antibody, the negative control and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m3G</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m3G (cat. No. C15200239). Cells were fixed with 4% formaldehyde for 10 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA and 0.05% Tween20. The cells were immunofluorescently labeled with the m3G antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m3G</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 10 µg of the Diagenode monoclonal antibody against m3G (cat. No. C15200239) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 (left) shows the Bioanalyzer profile obtained with the input RNA (top), the m3G antibody (middle) and the negative control (bottom). The position of the small, 18s and 28s RNA in the input is indicated (1, 2 and 3, respectively). The right figure shows the gel image for the m3G antibody, the negative control and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m3G</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m3G (cat. No. C15200239). Cells were fixed with 4% formaldehyde for 10 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA and 0.05% Tween20. The cells were immunofluorescently labeled with the m3G antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m3G</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 10 µg of the Diagenode monoclonal antibody against m3G (cat. No. C15200239) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 (left) shows the Bioanalyzer profile obtained with the input RNA (top), the m3G antibody (middle) and the negative control (bottom). The position of the small, 18s and 28s RNA in the input is indicated (1, 2 and 3, respectively). The right figure shows the gel image for the m3G antibody, the negative control and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m3G</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m3G (cat. No. C15200239). Cells were fixed with 4% formaldehyde for 10 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA and 0.05% Tween20. The cells were immunofluorescently labeled with the m3G antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m3G</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 10 µg of the Diagenode monoclonal antibody against m3G (cat. No. C15200239) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 (left) shows the Bioanalyzer profile obtained with the input RNA (top), the m3G antibody (middle) and the negative control (bottom). The position of the small, 18s and 28s RNA in the input is indicated (1, 2 and 3, respectively). The right figure shows the gel image for the m3G antibody, the negative control and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m3G</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m3G (cat. No. C15200239). Cells were fixed with 4% formaldehyde for 10 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA and 0.05% Tween20. The cells were immunofluorescently labeled with the m3G antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m3G</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 10 µg of the Diagenode monoclonal antibody against m3G (cat. No. C15200239) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 (left) shows the Bioanalyzer profile obtained with the input RNA (top), the m3G antibody (middle) and the negative control (bottom). The position of the small, 18s and 28s RNA in the input is indicated (1, 2 and 3, respectively). The right figure shows the gel image for the m3G antibody, the negative control and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m3G</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m3G (cat. No. C15200239). Cells were fixed with 4% formaldehyde for 10 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA and 0.05% Tween20. The cells were immunofluorescently labeled with the m3G antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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