Diagenode

2,2,7-trimethylguanosine monoclonal antibody

Catalog Number
Format
Price
C15200239
100 µg
$0.00
  Bulk order

Other names: N2,N2,7-Trimethylguanosine, m2,2,7G, m3G, TMG

Monoclonal antibody raised in mouse against 2,2,7-trimethylguanosine (m3G) conjugated to KLH.

Lot001
Concentration1 μg/μl
Species reactivityHuman, mouse, other (wide range): positive.
TypeMonoclonal
PurityProtein G purified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C.
Storage BufferPBS containing 50% glycerol, does not contain a preservative.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
IP 10 μg per IP Fig 1
IF 1:1,000 Fig 2
  • Validation data
    IP

    Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m3G
    Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 10 µg of the Diagenode monoclonal antibody against m3G (cat. No. C15200239) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 (left) shows the Bioanalyzer profile obtained with the input RNA (top), the m3G antibody (middle) and the negative control (bottom). The position of the small, 18s and 28s RNA in the input is indicated (1, 2 and 3, respectively). The right figure shows the gel image for the m3G antibody, the negative control and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    ELISA

    Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m3G
    HeLa cells were stained with the Diagenode monoclonal antibody against m3G (cat. No. C15200239). Cells were fixed with 4% formaldehyde for 10 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA and 0.05% Tween20. The cells were immunofluorescently labeled with the m3G antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  •  Applications
    IP
    Immunoprecipitation Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  •  Documents
    2,2,7-trimethylguanosine monoclonal antibody DATASHEET
    Monoclonal antibody raised in mouse against 2,2,7-trimethylguanosine (m3G) conjugated to KLH.
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  •  Publications

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