2,2,7-trimethylguanosine Antibody

Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m3G
Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 10 µg of the Diagenode monoclonal antibody against m3G (cat. No. C15200239) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 (left) shows the Bioanalyzer profile obtained with the input RNA (top), the m3G antibody (middle) and the negative control (bottom). The position of the small, 18s and 28s RNA in the input is indicated (1, 2 and 3, respectively). The right figure shows the gel image for the m3G antibody, the negative control and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m3G
HeLa cells were stained with the Diagenode monoclonal antibody against m3G (cat. No. C15200239). Cells were fixed with 4% formaldehyde for 10 min at RT, permeabilized with 0.5% Triton X-100 for 10 min at RT and blocked with PBS containing 1% BSA and 0.05% Tween20. The cells were immunofluorescently labeled with the m3G antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.