Diagenode

Epigenomics Profiling Services

DNA Methylation Profiling Services

DNA-methylation -Diagenode

The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.

RRBS-Diagenode

Illumina Infinium Epic Array (850K)

  • Cost-effective with rapid turnaround time
  • Quantitative analysis of methylation sites in CpG, non-CpG, and CHH sites
  • Over 850,000 human CpG positions at single nucleotide resolution
  • Differentially methylated site analysis
  • Comprehensive services includes end-to-end bisulfite conversion, array hybridization, and analysis

Reduced representation bisulfite sequencing (RRBS)

RRBS provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a cost-effective method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.

  • CpG rich region (5-mC): single nucleotide resolution
  • Epigenetic biomarker discovery
  • Methylation patterns of CpG rich regions across the genome
  • Covers up to 4M CpG sites in Human

Whole genome bisulfite sequencing (WGBS)

WGBS is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina® platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. Genome-wide bisulfite sequencing is suitable for studying DNA methylation sites and their role in gene regulation

  • Genome-wide 5-mC: single nucleotide resolution
  • Epigenetic biomarker discovery
  • Methylation patterns of CpG, CHG, and CHH regions across the genome
  • Epigenome-wide association studies (EWAS)

MeDIP/hMeDIP-seq

Targeted DNA methylation analysis


Products

Cat. No.ProductFormatPrice
G02020000 DNA Methylation Profiling (RRBS Service)
*Offer valid only until December 31, 2019. Customer must provide a minimum of 8 samples. Samples must be provided in increments of 8. Alignment to ref...
G02090000 Infinium Methylation EPIC array service
The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allow...
G02050000 MeDIP-seq service (Methylated DNA IP Sequencing)
Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) is used to study 5-mC genome-wide. In this assay, specific antibodies against 5-mC are used to capt...
G02070000 Targeted DNA methylation service
Targeted DNA methylation analysis allows for the quantitative study of cytosine methylation at known and precise locations throughout the genome. Depending o...
G02040000 WGBS service (Whole Genome Bisulfite Sequencing)
Whole-genome bisulfite sequencing (WGBS) is the most comprehensive next generation sequencing  for DNA methylation profiling, allowing single-base resol...
G02100000 Bioinformatics Data Mining Service
New! Data mining using machine learning (AI) for unique epigenetic data insights WHITE PAPERS Smokers vs non-smokers Breast cancer ...

Events

  • A Century of Genetics
    Edinburgh
    Nov 13-Nov 15, 2019
  • EMEA User Group Meeting - PacBio
    Barceló Hotel, Milan, Italy
    Nov 14-Nov 15, 2019
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