Diagenode

Epigenomics Profiling Services

DNA Methylation Profiling Services

DNA-methylation -Diagenode

The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.

RRBS-Diagenode

Reduced representation bisulfite sequencing (RRBS)

RRBS provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a cost-effective method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.

  • CpG rich region (5-mC): single nucleotide resolution
  • Epigenetic biomarker discovery
  • Methylation patterns of CpG rich regions across the genome
  • Covers up to 4M CpG sites in Human

Whole genome bisulfite sequencing (WGBS)

WGBS is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina® platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. Genome-wide bisulfite sequencing is suitable for studying DNA methylation sites and their role in gene regulation

  • Genome-wide 5-mC: single nucleotide resolution
  • Epigenetic biomarker discovery
  • Methylation patterns of CpG, CHG, and CHH regions across the genome
  • Epigenome-wide association studies (EWAS)

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Superior coverage for DNA methylation studies: fast and affordable Superior coverage– 4 million CpGs High throughput - 96 samples processed in ...

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