ChIP-seq results from 3D cell culture showed equivalent reproducibility to 2D cell culture for H3K4me3 and H3K27ac. However, 3D cell culture is advantageous over 2D cell culture with:
ChIP-Seq experiment performed on HepG2 cells cultivated in adherent condition with traditional 2D culture plates and in spheroids with our new 3D micro pattern culture plates, using H3K4me3 and H3K27ac targets. The 2D and 3D figures are nearly identical with equivalent peaks and read numbers, highlighting comparability to standard culture. Results from 3D cell culture, however, provide additional benefits such as stronger enrichment and higher physiological relevance from spheroid cells.
Diagenode offers a revolutionary solution to enhance your epigenetics research using cultured cells. Our new 3D micro pattern cell culture plates give physiological relevance to epigenetic experiments. Unlike 2D monolayer culture, 3D culture plates enable spheroid culture that favors natural cell-cell interaction and mimics the in vivo environment.
These ready-to-use and standardized plates are optimal for reproducible cell-based ChIP-seq assays as well as microscopic imaging, toxicology assays, and drug development.
|2D||Low binding affinity|
|3D||3D Diagenode micro pattern culture plate|
|Disease||Cell line||ATCC collection reference||2D Monolayer 100x||3D 40x||3D 100x|
HepG cells cultivated on our new 3D micro pattern cell culture plates. Chromatin was sheared according to iDeal ChIP-seq kit procedure.
H3K4me Western blot performed on chromatin isolated from 3D micro pattern culture of HepG cells.
WCL = whole cell lysate of HeLa cells.
3D cell culture optimizes the ability for cell differentiation compared to 2D
Human mesenchymal UET-13 stem cells cultivated on 3D micro pattern culture plate shows increased ability to differentiate to adipocyte compared with 2D. Lipid-droplet stained by Oil red O.
Marker gene expressions showed higher efficiency of differentiation into mature adipocyte expressing Adiponectin, PPARγ and C/EBPα.
Miyagawa et al, Tissue Eng Part A. (2011)