Bioruptor® protocol update: Procedure for the purification of proteins with the Bioruptor® for TAP-TAG experiments The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complexes. The fusion of the TAP tag to the protein of interest allows the rapid purification under a native environment. Molecular complexes can then be isolated and used for various applications for the identification of partners. Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes combined the TAP tagging with the Bioruptor® sonication device. This combination is essential for the efficient purification of a chromatin protein. Download New publication using Bioruptor®: Antigen I/II role highlight in biofilm formation and fibrinogen binding Pathogens like streptococci secrete surface proteins promoting the adhesion and stimulating the colonization of the oral cavity of humans and animals. Sarah Chuzeville and collaborators used the Bioruptor® to release cytoplasmic proteins allowing the study of the antigens I/II expression, the multimodal adhesins identified on Integrative and Conjugative Elements. Researchers used a bioinformatic approach and demonstrated the contribution of Integrative and Conjugative Elements to increase biofilm formation between species and their contribution for streptococci virulence. Read this Microbial Pathogenesis paper Creative use of the Bioruptor®: Making Nuclear Magnetic Resonance metabolomics more easy The visualisation of the metabolites contained in cells or tissues is a very powerful tool to understand how the local metabolism and biochemical pathways are affected by external or internal stimuli or pathologies. However, a robust, reproducible, and fast procedure that releases metabolites is required. Pascal de Tullio and his collaborators sought out an improved extraction of the methabolites method to be compatible with NMR analysis. Their research demonstrates that sonication with the Bioruptor®, a conventional tool for protein, DNA and RNA extraction, can be used to disrupt tissues or cells for metabolomic analysis. The rapidity and the simplicity of this approach represent an efficient alternative to other protocols and offer the opportunity for a high-throughput application in intracellular and intratissular metabolite measurements. Read the paper Europe - Diagenode s.a. orders@diagenode.com / info@diagenode.com North America - Diagenode Inc. orders.na@diagenode.com / info.na@diagenode.com