Conventional ChIP protocols require high numbers of cells (hundreds of thousands cells at least) limiting the application for ChIP technology to few cell samples. Recently, ChIP assays on smallest amount of cells have been reported. The procedure requires tedious optimization of several reaction conditions to face the increased background observed in ChIP performed with reduced amount of cells. That might consequently lead to considerable time and lab expenditures. To reduce these tedious steps, Diagenode provides the new Auto True MicroChIP kit with optimized reagents and protocol to enable successful ChIP on as few as 10 000 cells. Moreover, the Auto True MicroChIP kit protocol has been thoroughly optimised by Diagenode for ChIP followed by high-throughput sequencing on Illumina® Next-Gen sequencers. To allow the generation of consistent results in ChIP-seq on 10 000 cells, Diagenode has also optimised a library preparation protocol on limited amount of immunoprecipitated DNA.
The new MicroPLEX library preparation kit allows the preparation of libraries for sequencing on picogram amount of immunoprecipitated DNA. Thus, association of the Auto True MicroChIP kit with the MicroPLEX library preparation kit provides optimised solutions to perform ChIP-sequencing on limited amount of cells.