Protocols
Download Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing
Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation of most archived pathological specimens. Archival FFPE samples provide an invaluable repository of in...
Download Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using Bioruptor&...
Download Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation
Histology-defined proteomics investigations are a fundamental tool in modern molecular pathology. Molecular signatures of specific cells must be detected within their correct histopathological cont...
Download TruSeq™ Stranded RNA Sample Preparation - IP-Star® Compact
The “TruSeq Stranded RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ Stranded Total RNA Sample Preparation” kit or “TruSeq™ Str...
Download TruSeq™ RNA Sample Preparation - IP-Star Compact
The “TruSeq RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ RNA Sample Preparation” kit v2 and reagents from Illumina®. It provides fle...
Download TruSeq™ DNA PCR-Free Sample Preparation - IP-Star Compact
The “TruSeq™ DNA PCR-Free SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ DNA PCR-Free Sample Preparation” kit and reagents from Illumina...
Download TruSeq™ ChIP Sample Preparation - IP-Star® Compact
The “TruSeq ChIP SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ ChIP Sample Preparation” kit and reagents from Illumina®. It provides flex...
Download TruSeqTM ChIP Sample Preparation - IP-Star® Compact -
The “TruSeq ChIP SamplePrep” protocol on the IP-Star® is using the standard “TruSeqTM ChIP Sample Preparation” kit and reagents from Illumina®. It provides flexibili...
Download TruSeq DNA sample preparation - IP-Star® Compact
The “TruSeq DNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeqTM DNA Sample Preparation” kit and reagents from Illumina®. It provides flexibility...
Download Transfection of CRISPR/Cas9 Nuclease NLS ribonucleoprotein (RNP) into adherent mammalian cells using Lipofectamine® RNAiMAX
The CRISPR/Cas genome editing system consists of a single guide RNA (sgRNA) and the Cas9 endonuclease. To create gene disruptions, a single guide RNA is generated to direct the Cas9 nuclease to a s...
Download TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a nat...
Download RNA Shearing for Bioruptor® NGS and Pico
The Diagenode ACT (Adaptative Cavitation Transfer technology) process is highly reproducible, however attention must be paid to the following operating conditions to ensure best RNA shearing r...
Download RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit
Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disrup...
Download Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico
ChIP-seq is a common tool used to study protein-DNA interactions in cells and tissues on a genome-wide scale. The size of the immunoprecipitated DNA fragments is a key parameter for successful libr...
Download Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus
Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Effici...
Download Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Effici...
Download PCR amplicons shearing using the Bioruptor Pico
With the Bioruptor® Pico and the former model Bioruptor® NGS it is possible to shear PCR amplicons of at least 500 bp in size. While PCR fragments bigger than 700 bp are sheared accord...
Download NEBNext® ChIP-Seq Library Prep for Illumina - IP-Star Compact
The “NEBNext ChIP-Seq Illumina” protocol on the IP-Star® is using the standard “NEBNext® ChIP-Seq Library Prep for Illumina” kit and reagents from NEB®. This kit...
Download NEBNext® ChIP-Seq Library Prep for Illumina - IP-Star® Compact -
The “NEBNext_ChIP-Seq_Illumina” protocol on the IP-Star® is using the standard “NEBNext® ChIP-Seq Library Prep for Illumina” kit and reagents from NEB®. This kit...
Download MicroPlex Library Preparation kit™ v2 - IP-Star Compact
The “MicroPlex Library Preparation” protocol on the IP-Star® is using the standard “MicroPlex Library Preparation™ kit” and reagents from Diagenode. The MicroPlex ...