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Infinium MethylationEPIC and Hydroxymethylation oxEPIC Array Service

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  •  描述
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目录号
格式
G02090004

The Infinium MethylationEPIC & Hydroxymethylation oxEPIC Array utilizes a genome-wide DNA methylation analysis technique based on selective oxidation, bisulfite conversion, and Illumina® technology. The technique allows to quantitively detect the true methylation (5mC) and total methylation (5mC + 5hmC) levels of over 850,000 CpG positions throughout a human genome at single nucleotide resolution.

Briefly, upon bisulfite treatment, both 5‑methylcytosine and 5-hydroxymethylcytosine are read as cytosines, giving a map of total methylation (5mC + 5hmC) level in the DNA sample. Alternatively, by performing first an oxidation step, 5-hydroxymethylcytosine is converted to 5-formylcytosine, which subsequently converts to uracil during bisulfite treatment. Consequently, only 5‑methylcytosine is read as cytosine, giving a map of the true methylation (5mC) level in the DNA sample. Combining both methods enables the interrogation of 5-hydroxymethylcytosine (5hmC) status in the DNA sample.

See our Infinium MethylationEPIC Array Service if only interested in the total methylation (5mC + 5hmC) level in the DNA sample

Excellent method for comprehensive genome-wide coverage

  • Cost-effective with rapid turnaround time
  • Over 850,000 CpGs detected in human samples at single nucleotide resolution
  • Quantitative interrogation of CpG, non-CpG, and CHH sites
  • Discrimination between 5mC and 5hmC
  • Differentially methylated and hydroxymethylated site analysis
  • Comprehensive services include end-to-end oxidative conversion, bisulfite conversion, array hybridization, and analysis

See our other DNA methylation profiling services for reduced representation, whole genome, and targeted analysis

  • Description
    Infinium MethylationEPIC and Hydroxymethylation oxEPIC Services

    End-to-end array

    • Oxidative conversion
    • Bisulfite conversion
    • Whole genome amplification  
    • Array hybridization
    • Single base extension  
    • Array scanner 
  • Bioinformatic analysis

    Analysis

    Features

    Standard

    Standard files provided:

    • Sample annotation
    • Variable annotation
    • Scanner output (IDAT files)

    Differential methylation and hydromethylation analysis

    Identification of differentially methylated and hydroxymethylated CpGs between sample groups.

     Files provided:

    • Report with summary of differential methylation and hydroxymethylation analysis and plots
    • File containing the differentially methylated and hydroxymethylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
    • File containing differential methylated regions (DMRs) and differentially hydroxymethylated regions (DHMRs)

    Gene ontology terms analysis

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.

    Pathway analysis

    Identify biochemical pathways in which genes associated with differentially methylated and hydroxymethylated regions (or individual differentially methylated and hydroxymethylated CpGs) may be overrepresented.

  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Infinium MethylationEPIC and Hydroxymethylation oxEPIC Array Service (Diagenode Cat# G02090004). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  相关产品

活动

  • ChIP workshop
    Diagenode
    Sep 21-Sep 22, 2021
  • DNA methylation workshop: Master MeDIP assay
    Diagenode
    Sep 30, 2021
  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
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