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High Throughput 3’mRNA-seq Service

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  •  描述
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目录号
格式
G02140000
Custom

The High Throughput 3’mRNA-seq is an attractive solution to perform comparative gene expression analysis on a large number of samples. It utilizes the proprietary technology Mercurius which enables reliable gene expression quantification for large investigational studies. This approach is optimal for assessing multiple conditions of interest or increasing statistical power underlying any differential expression analysis with larger sets of replicates.

Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of 3’mRNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on Mercurius protocol which uses early multiplexing to produce Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.

Scalable and affordable 3’mRNA-seq

  • Reliable gene expression quantification
  • Accurate comparison of gene expression levels
  • Handling large number of samples simultaneously
  • Working with very low-quality RNA samples
  • Fast turnaround time (from 4 to 6 weeks)

  • Sample Requirements
    • Project size: minimum of 96 samples
    • Sample type: total isolated RNA
    • Optimal concentration: ≥ 20 ng/µL
    • Optimal volume: ≥10 µL
    • Shipment conditions: shipped in 96-well plates (low bind, RNase free, Eppendorf, 0030129504)
    • Batch effect: All the RNA samples intended to be used for the differential expression analysis should be included in the same library, bearing up to 96 samples
  • Services Workflow

    What our service includes

    1. RNA quality control (optional for additional fee)
      • Determination of RIN value (RNA integration number)
      • Measurement of RNA concentration
    2. Multiplexed library preparation
      • Reverse transcription
      • Sample multiplexing
      • Stranded library construction
      • Quality control of pooled libraries prior to sequencing
    3. Sequencing on Illumina platforms
      • 4 million raw reads per sample
    4. Data analysis (optional for additional fee)
      • Standard bioinformatic analysis
      • Advanced bioinformatic analysis
      • Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request
  • Bioinformatic Analysis

    Standard bioinformatic analysis
    (optional for additional fee)

    Advanced bioinformatic analysis
    (optional for additional fee)

    • Demultiplexing, read quality control, trimming and filtering
    • Alignment to reference genome
    • Quantification of known transcripts

    Provided files:

    • Report with sequencing statistics
    • Raw data in FASTQ format
    • FastQC report
    • Alignment in BAM format
    • Un-normalized read counts
    • Differential expression analysis
    • Gene ontology terms analysis
    • Pathway analysis

  •  文档
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: High Throughput 3’mRNA-seq Service (Diagenode Cat# G02140000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing
    Daniel Alpern, Vincent Gardeux, Julie Russeil, Bastien Mangeat, Antonio C. A. Meireles-Filho, Romane Breysse, David Hacker and Bart Deplancke
    Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq ha...

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