The High Throughput 3’mRNA-seq is an attractive solution to perform comparative gene expression analysis on a large number of samples. It utilizes the proprietary technology Mercurius which enables reliable gene expression quantification for large investigational studies. This approach is optimal for assessing multiple conditions of interest or increasing statistical power underlying any differential expression analysis with larger sets of replicates.
Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of 3’mRNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on Mercurius protocol which uses early multiplexing to produce Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
Batch effect: All the RNA samples intended to be used for the differential expression analysis should be included in the same library, bearing up to 96 samples
Diagenode strongly recommends using this: High Throughput 3’mRNA-seq Service (Diagenode Cat# G02140000). Click here to copy to clipboard.
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BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing Daniel Alpern, Vincent Gardeux, Julie Russeil, Bastien Mangeat, Antonio C. A. Meireles-Filho, Romane Breysse, David Hacker and Bart Deplancke Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq ha...
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