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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&Tag</strong> technologies.</p>',
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<li>flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&Tag</strong> technologies.</p>',
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<li>flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:</p>
<p><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">24 SI for ChIPmentation, Cat. No. C01011031</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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<ul>
<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div id="video"></div>
<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> – The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> – <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> – <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
<div class="extra-spaced">
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<div class="small-12 medium-4 large-4 columns"><center><a href="https://www.citeab.com/awards/2018/innovative-product-of-the-year" target="_blank"><img src="https://www.diagenode.com/emailing/images/citeab.png" width="224" height="200" /></a><br /><br />
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star® to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> µChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<h6 style="height:60px">TAG Kit for ChIPmentation</h6>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:</p>
<p><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">24 SI for ChIPmentation, Cat. No. C01011031</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
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<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
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<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&Tag</strong> technologies.</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:</p>
<p><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">24 SI for ChIPmentation, Cat. No. C01011031</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> – The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> – <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> – <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
<div class="extra-spaced">
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
</div>
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<div class="small-12 medium-4 large-4 columns"><center><a href="https://www.citeab.com/awards/2018/innovative-product-of-the-year" target="_blank"><img src="https://www.diagenode.com/emailing/images/citeab.png" width="224" height="200" /></a><br /><br />
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star® to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> µChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<h6 style="height:60px">TAG Kit for ChIPmentation</h6>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:</p>
<p><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">24 SI for ChIPmentation, Cat. No. C01011031</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
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<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&Tag</strong> technologies.</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
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<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:</p>
<p><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">24 SI for ChIPmentation, Cat. No. C01011031</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> – The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> – <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> – <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<div class="small-12 medium-4 large-4 columns"><center><a href="https://www.citeab.com/awards/2018/innovative-product-of-the-year" target="_blank"><img src="https://www.diagenode.com/emailing/images/citeab.png" width="224" height="200" /></a><br /><br />
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star® to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> µChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<h6 style="height:60px">TAG Kit for ChIPmentation</h6>
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:</p>
<p><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">24 SI for ChIPmentation, Cat. No. C01011031</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&Tag</strong> technologies.</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:</p>
<p><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">24 SI for ChIPmentation, Cat. No. C01011031</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> – The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> – <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> – <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<div class="small-12 medium-4 large-4 columns"><center><a href="https://www.citeab.com/awards/2018/innovative-product-of-the-year" target="_blank"><img src="https://www.diagenode.com/emailing/images/citeab.png" width="224" height="200" /></a><br /><br />
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<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
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<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star® to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> µChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<div class="small-12 columns"><center><img src="../../img/product/kits/chipmentation-sequencing-p65.png" /></center></div>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> TAG Kit for ChIPmentation</strong> 添加至我的购物车。</p>
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<h6 style="height:60px">TAG Kit for ChIPmentation</h6>
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The <strong>TAG Kit for ChIPmentation</strong> has been developed for researchers who would like to perform ChIPmentation using their own validated ChIP protocol. Our TAG Kit for ChIPmentation includes reagents for <strong>tagmentation-based library preparation</strong> and is compatible with any ChIP protocol based on magnetic beads. The <strong>primer</strong> <strong>indexes for multiplexing</strong> must be <strong>purchased separately</strong> and are available as a reference:</p>
<p><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">24 SI for ChIPmentation, Cat. No. C01011031</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137<br /></a><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank"><br /></a></p>',
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<ul>
<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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