Assessment of hydroxymethylated DNA based on glucosylation and restriction.
It has been shown that β-glucosyltransferase can be used to discriminate between 5-mC and 5-hmC bases using glucosyl-sensitive restriction enzyme such a...
PvuRts1l restriction enzyme
PvuRts1I is a restriction enzyme that selectively cleaves 5-hmC-containing DNA sequences. PvuRts1I is able not only to discriminate between 5-hmC and 5-mC bu...