Diagenode

Protein Analysis

Protein extraction

Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Diagenode's Bioruptor® deliver high quality protein.

Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).

Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).

Made for Protein extraction

  • Bioruptor-pico-next-gen-sequencing
    B01060001
    Bioruptor® Pico sonication device
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    C30010016
    1.5 ml Bioruptor® Pico Microtubes with Caps
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    C30010010-1000
    1.5 ml Bioruptor® Plus TPX microtubes
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    C30010010-50
    1.5 ml Bioruptor® Plus TPX microtubes
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    B01200040
    1.5 ml tube holder for Bioruptor® Pico
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    B01200011
    1.5 ml tube holder for Bioruptor® Standard ...
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    B01200012
    10 ml tube holder for Bioruptor® Standard & Plus
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    C30010009
    15 ml Bioruptor® Plus TPX tubes
  • 15 ml tubes
    C30010017
    15 ml Bioruptor® Pico Tubes
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    B01200016
    15 ml sonication accessories for Bioruptor®...
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    B01200013
    15 ml tube holder for Bioruptor® Standard &...
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    B01200014
    50 ml tube holder for Bioruptor® Standard &...
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    B01020001
    Bioruptor® Plus sonication device
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    C20000021
    Protein Extraction beads
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    C20000020
    Protein Extraction kit for Bioruptor® Plus

Related protocols

Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...
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TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein com...
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Activity Test - Procedure for immunoprecipitation of native chromatin proteins
Proteins play role in almost all cellular processes, and the study of their activity is crucial f...
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Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
The regular protocol for the extraction of histone requires an acid extraction, making impossible...
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Mass-Spectrometry: procedure for shotgun proteomics
Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Sh...
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Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis
Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck ...
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Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
  • XXXIX Reunión anual de la Sociedad de Bioquimica y Biología Molecular de Chile
    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
 See all events

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