Diagenode

Next Generation Sequencing

Library preparation

Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing

After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.

Diagenode offers a comprehensive product portfolio for library preparation:

CATS RNA-seq Library Preparation Kits

Diagenode’s new RNA-sequencing solutions utilize the innovative “Capture and Amplification by Tailing and Switching” (CATS), a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. Learn more

ChIP-seq and DNA sequencing library preparation solutions

Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our kits are an optimal choice for library preparation from DNA inputs down to 50 pg. Learn more

Bioruptor Pico - short fragments

Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions  between 150bp -2kb. Learn more

Megaruptor® - long fragments

The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing.  Learn more

Made for Library preparation

Cat. No.ProductFormatPrice
C05010043 CATS mRNA-seq Kit (with polyA selection)
Diagenode’s CATS mRNA-seq Kit (with polyA selection) utilizes the innovative “capture and amplification by tailing and switching” (CATS), a...
24 rxns $1,395.00
C05010040 CATS Small RNA-seq Kit
Diagenode’s CATS Small RNA-seq Kit utilizes the innovative “capture and amplification by tailing and switching” (CATS), a ligation-free met...
24 rxns $1,360.00
C05010042 CATS Total RNA-seq Kit (with rRNA depletion)
Diagenode’s CATS Total RNA-seq Kit (with rRNA depletion) utilizes the innovative “capture and amplification by tailing and switching” (CATS...
24 rxns $2,450.00
C05010020 iDeal Library Preparation Kit x24 (incl. Index Primer Set 1)
The iDeal Library Preparation Kit reliably converts DNA into indexed libraries for next-generation sequencing, with input amounts down to 1 ng. Our kit offer...
24 rxns $890.00
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit
C05010012 MicroPlex Library Preparation Kit v2 (12 indices)
Specifically optimized for ChIP-seqThe MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allow...
12 rxns $1,100.00
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc $35.00
C17011050-50 CATS paired-end sequencing primer
This primer has been specifically designed to be used in paired-end sequencing with CATS RNA-seq libraries on illumina® platforms (MiSeq, HiSeq)
50 µl $30.00
C05010041 CATS RNA-seq Kit
Diagenode’s CATS RNA-seq Kit utilizes the innovative “capture and amplification by tailing and switching” (CATS), a ligation-free method to...
24 rxns $1,360.00
C05010021 Index Primer Set 2 (iDeal Library Preparation Kit x24)
The iDeal Library Preparation kit reliably converts DNA into indexed libraries for next-generation sequencing, with input amounts down to 1 ng. Our kit offer...
24 rxns $195.00
C05010014 MicroPlex Library Preparation Kit v2 (48 indices)
Specifically optimized for ChIP-seqThe MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allow...
48 rxns $4,300.00

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